Apoptotic and anti-proliferative effects of 17β-estradiol and 17β- estradiol -like compounds in the Hep3B cell line

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题名:	Apoptotic and anti-proliferative effects of 17β-estradiol and 17β- estradiol -like compounds in the Hep3B cell line
作者:	(Erh-Jung Huang);(Cheng-Chung Wu);(Hsien-Ping Huang);(Jer-Yuh Liu);(Chung-Sheng Lin);(Jaung-Geng Lin);(Li-Mien Chen);李信達(Shin-Da Lee);郭薇雯(Wei-Wen Kuo);黃志揚(Chih-Yang Huang)*
贡献者:	醫學院基礎醫學研究所
关键词:	hepatocellular carcinoma (HCC);17 β-estrodiol;17 β-estrodiol-like compounds;apoptotic and antiproliferative effects
日期:	2006-10
上传时间:	2009-08-26 16:09:38 (UTC+8)
摘要:	Since there is evidence for estrogen and estrogen-like compounds to have beneficial effect on the pathogenesis of hepatocellular carcinoma (HCC), this study was designed to investigative the apoptotic and anti-proliferative effects of these compounds on the human hepatoma Hep3B cell line. The Hep3B cells were treated with 17β-estradiol (E2), diethylstilbestrol (DES), tamoxifen, and genistein. After treatments of these compounds at the concentration of 10-6 or 10-8 M, the Hep3B cells were demonstrated to have significant DNA fragmentation, nucleus condensation, cytochrome-c leaking from the mitochondria and caspase-3 activation by DAPI and Western blotting. The cells were also observed to have declined proliferative potential by MTT assay, arrested cell cycle by flow-cytometry measurements. However, the cytochrome-c leaking from the mitochondria induced by E2 and E2-like compounds was blocked totally by ICI 182,780 treatment. These finding suggest that estrogen and the estrogen-like compounds may induce anti-proliferative and apoptotic effects in Hep3B cells, and the E2 and the E2-like compounds mediated apoptotic effect was estrogen receptor dependent. Among the drugs tested, E2, E2 agonists (DES and genistein) and partial antagonist (tamoxifen), all showed the stronger anti-tumor potential.? Since there is evidence for estrogen and estrogen-like compounds to have beneficial effect on the pathogenesis of hepatocellular carcinoma (HCC), this study was designed to investigative the apoptotic and anti-proliferative effects of these compounds on the human hepatoma Hep3B cell line. The Hep3B cells were treated with 17β-estradiol (E2), diethylstilbestrol (DES), tamoxifen, and genistein. After treatments of these compounds at the concentration of 10-6 or 10-8 M, the Hep3B cells were demonstrated to have significant DNA fragmentation, nucleus condensation, cytochrome-c leaking from the mitochondria and caspase-3 activation by DAPI and Western blotting. The cells were also observed to have declined proliferative potential by MTT assay, arrested cell cycle by flow-cytometry measurements. However, the cytochrome-c leaking from the mitochondria induced by E2 and E2-like compounds was blocked totally by ICI 182,780 treatment. These finding suggest that estrogen and the estrogen-like compounds may induce anti-proliferative and apoptotic effects in Hep3B cells, and the E2 and the E2-like compounds mediated apoptotic effect was estrogen receptor dependent. Among the drugs tested, E2, E2 agonists (DES and genistein) and partial antagonist (tamoxifen), all showed the stronger anti-tumor potential.
關聯:	MOLECULAR AND CELLULAR BIOCHEMISTRY 290(1-2)1 ~7
显示于类别:	[基礎醫學研究所] 期刊論文

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