Cloning and Characterization of an Outer Membrane Protein (Lip18) from Helicobacter bizzozeronii

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 生命科學院 > 生物科技學系暨碩士班 > 期刊論文 > Item 310903500/6995

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题名:	Cloning and Characterization of an Outer Membrane Protein (Lip18) from Helicobacter bizzozeronii
作者:	(Jiaqian Zhu);徐媛曼;張照夫(Chao-Fu Chang);張清棟(Ching-Dong Chang);(Kenneth W. Simpson);(Huolin Tu);張永富(Yung-Fu Chang)*
贡献者:	醫學院生物科技學系
日期:	2003-04
上传时间:	2009-08-26 16:07:13 (UTC+8)
摘要:	A recombinant lambda ZapII phage was selected by screening a genomic library of Helicobacter bizzozeronii (Hb) using antibodies from a naturally infected cat. DNA sequencing resulted an open reading frame containing 172 codons with a predicted molecular mass of 18 kD (Lip18). The amino acid sequence showed 22.1%, 55.2%, 56.7% and 57.1% identity to peptidoglycan-associated lipoprotein of H. pylori, Agrobacterium tumefaciens, Bartonella bacilliformis, respectively. A peptidoglycan associating a-helical motif (LALGQRRSVAVRDYLVS) was located in the C-terminal region. H. bizzozeronii contains a potential lipoprotein signal peptide cleavage site (Val-Val-Gly-Cys), and yields a predicted mature protein with 148 amino acids. The Lip18 was localized into the outer membrane of the bacteria. Immunoblot analysis of serum samples from a dog and cat naturally infected with Helicobacter spp was able to recognize the purified recombinant Lip18.
關聯:	DNA SEQUENCE 14(2 )87 ~94
显示于类别:	[生物科技學系暨碩士班] 期刊論文

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