Modified nitrocefin-EDTA method to differentially quantify the induced L1 and L2 beta-lactamase in Stenotrophomonas maltophilia

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 醫學院 > 醫學檢驗生物技術學系暨碩士班 > 期刊論文 > Item 310903500/6785

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题名:	Modified nitrocefin-EDTA method to differentially quantify the induced L1 and L2 beta-lactamase in Stenotrophomonas maltophilia
作者:	胡若梅(Rouh-Mei Hu);蔣凱弘(Kai-Hung Chiang);林振文(Lin Cheng Wen);楊翠青(Tsuey-Ching Yang)*
贡献者:	健康照護學院醫學檢驗生物技術學系;中國附醫檢驗醫學部
关键词:	β-lactamase;ethylene diamine tetraacetic acid;L1;nitrocefin;Stenotrophomonas maltophilia
日期:	2008-10
上传时间:	2009-08-26 16:00:28 (UTC+8)
摘要:	Aim: To estimate the ethylene diamine tetraacetic acid (EDTA) concentration at which the L1 enzyme activity in the cell extracts of Stenotrophomonas maltophilia can be mostly inhibited. Methods and Results: The effective inhibition concentration of EDTA against the L1 enzyme in the cell extracts was firstly evaluated by using the L2 isogenic mutant of S. maltophilia KJ, KJΔL2, as the assayed strain. Approximately 92% L1 activity was inhibited by 10 mmol l−1 EDTA, which is 100-fold higher than that from previously reported protocols (0·1 mmol l−1). Three phylogenetic clusters of L1 proteins were revealed from 11 clinical S. maltophilia isolates, with a L1 protein divergence of 0–11%. The EDTA concentration required to inhibit the L1 enzymes of different phylogenetic clusters was estimated to be 10 mmol l−1. Conclusion: The previous nitrocefin-EDTA protocol for differentially quantifying the L1 and L2 activity in the cell extracts has been modified by raising the added EDTA concentration to 10 mmol l−1. Significance and Impact of the Study: A rapid and accurate method for determination of L1 and L2 activity will provide a convenient tool for enzyme characterization and induction mechanism study of S. maltophilia.
關聯:	LETTERS IN APPLIED MICROBIOLOGY 47(5)457~461
显示于类别:	[醫學檢驗生物技術學系暨碩士班 ] 期刊論文

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