Monoclonal antibodies against human ribosomal P proteins penetrate into living cells and cause apoptosis of Jurkat cells in culture. (SCI)

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Title:	Monoclonal antibodies against human ribosomal P proteins penetrate into living cells and cause apoptosis of Jurkat cells in culture. (SCI)
Authors:	(Kuang-Hui Sun)*;(Shye-Jye Tang);林孟亮(Meng-Liang Lin);(Yu-Shan Wang);(Guang-Huan Sun);(Wu-Tse Liu)
Contributors:	健康照護學院醫學檢驗生物技術學系
Date:	2001.01
Issue Date:	2009-08-26 15:59:41 (UTC+8)
Abstract:	OBJECTIVE: This study was designed to determine the role of autoantibodies to the ribosomal P protein (anti-P Abs) in the pathogenesis of systemic lupus erythematosus (SLE) using monoclonal anti-P antibodies (anti-P mAbs). METHODS: Anti-P mAbs were prepared by a standard hybridoma procedure using recombinant human P1 and P2 proteins as immunogens. We studied the reactivities of these mAbs to P proteins, their binding and penetration capabilities in different cell lines and their apoptotic effects on Jurkat T cells. RESULTS: In addition to recognizing human P0, P1 and P2 proteins, the anti-P mAb 9B6-4 bound to 20-40% and penetrated 50-90% of astrocytes, Jurkat T cells and lung cancer cells via the P0 surface protein. Treatment with the mAb 9B6-4 also caused increases in the percentages of Jurkat T cells in the sub-G1 phase of the cell cycle (14.8%) and undergoing apoptosis (21.3%). CONCLUSION: Anti-P autoantibodies may play a role in the pathogenesis of lymphopenia or lymphocyte dysfunction in SLE.
Relation:	RHEUMATOLOGY 40(7 )750 ~756
Appears in Collections:	[Department of Medical Laboratory Science and Biotechnology ] Journal articles

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