Protective effect of alpha-keto-beta-methyl-n-valeric acid on BV-2 microglia under hypoxia or oxidative stress

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 健康照護學院 > 物理治療學系(停用) > 期刊論文 > Item 310903500/6574

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题名:	Protective effect of alpha-keto-beta-methyl-n-valeric acid on BV-2 microglia under hypoxia or oxidative stress
作者:	黃雪美(Huang HM);歐秀中(Hsiu-Chung Ou);陳奐亮;侯建維(Huo RC);鄭啟清(Jeng KC)*
贡献者:	健康照護學院物理治療學系
关键词:	hypoxia;oxidative stress;reactive oxygen species;cell viability;KMV;caspase-3;caspase-9
日期:	2005-05
上传时间:	2009-08-26 15:54:53 (UTC+8)
摘要:	The alpha-ketoglutarate dehydrogenase complex (KGDHC) is a mitochondrial enzyme in the TCA cycle. Inhibition of KGDHC activity by alpha-keto-beta-methyl-n-valeric acid (KMV) is associated with neuron death. However, the effect of KMV in microglia is unclear. Therefore, we investigated the effect of KMV on BV-2 microglial cells exposed to hypoxia or oxidative stress. The results showed that KMV (1-20 mM) enhanced the cell viability under hypoxia. KMV dose-dependently reduced ROS and LDH releases from hypoxic BV-2 cells. KMV also reduced ROS production and enhanced the cell viability under H2O2 but failed to reduce the SIN-1 and sodium nitroprusside (SNP) toxicity. KMV also reduced caspase-3 and -9 activation under stress. These results suggest that KMV protects BV-2 cells from stress and acts by reducing ROS production through inhibition of KDGHC.
關聯:	ANNALS OF THE NEW YORK ACADEMY OF SCIENCES (1042 )272 ~278
显示于类别:	[物理治療學系(停用)] 期刊論文

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