中國醫藥大學機構典藏 China Medical University Repository, Taiwan:Item 310903500/6324
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    题名: Contrasting selection modes at Adh1 locus in outcrossing Miscanthus sinensis vs. inbreeding Miscanthus condensatus (Poaceae)
    作者: 周昌弘(Chang-Hung Chou)
    贡献者: 生命科學院生態暨演化生物學研究所;中國附醫院長室
    关键词: alcohol dehydrogenase 1;mating systems;Miscanthus condensatus;Miscanthus sinensis;modes of selection;Poaceae;population demography
    日期: 2003
    上传时间: 2009-08-25 14:41:05 (UTC+8)
    摘要: We estimated DNA sequence variation of the Adh1 locus in the outcrossing Miscanthus sinensis (Poaceae) and its close selfing relative, M. condensatus. Tajima's test of selection is significantly negative for both overall exons and replacement sites in M. sinensis. Among its entire sample, nucleotide diversity of nonsynonymous sites is higher than that of synonymous sites. A McDonald and Kreitman test of neutrality indicates an excess of intraspecific replacement polymorphisms, suggesting possible directional selection toward advantageous mutants. However, frequent intragenic recombination suggests both purifying and positive selection is unlikely. Recent demographic expansions coupled with relaxation of purifying selection may have resulted in elevated genetic diversity at the Adh1 locus as well as the trnL-trnF intergenic spacer of cpDNA in this outcrossing species. In contrast, low levels of genetic diversity were detected at both the Adh1 locus and the cpDNA spacer in M. condensatus, consistent with bottlenecks associated with selfing in all populations. While Tajima's D and Fu and Li's F statistics did not reveal deviation from neutrality at the Adh1 locus in M. condensatus, 12 replacements vs. 10 synonymous changes were detected. Based on pairwise comparisons of the dN/dS ratio, lineages of closely related populations of the species distributed along saline habitats appeared to be under directional selection.
    關聯: AMERICAN JOURNAL OF BOTANY 90(4)561 ~570
    显示于类别:[生態暨演化生物學研究所 ] 期刊論文

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