Baicalein Induces Apoptosis in SCC-4 Human Tongue Cancer Cells via a Ca2+-dependent Mitochondrial Pathway

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Title:	Baicalein Induces Apoptosis in SCC-4 Human Tongue Cancer Cells via a Ca2+-dependent Mitochondrial Pathway
Authors:	(YUH-TZY LIN)、楊家欣(Yang Jai-Sing)、(HUI-JU LIN)、譚思濰(Tan Tzu-Wei)、(NOU-YING TANG)、(JO-HUA CHAING)、張永賢(Yung-Hsien Chang)、(HSU-FENG LU)、鍾景光(Jing-Gung Chung)*
Contributors:	生命科學院生物科技學系;中國附醫醫學研究部
Keywords:	Baicalein;reactive oxygen species;cytochrome c;cytoplasmic Ca2+;caspase-3;mitochondrial death pathway;apoptosis;SCC-4 cells
Date:	2007.12
Issue Date:	2009-08-25 14:40:02 (UTC+8)
Abstract:	Background: The effects of baicalein on SCC-4 human tongue cancer cells were examined to better understand its effect on apoptosis and associated possible signal pathways in vitro. Materials and Methods: Apoptosis induction, reactive oxygen species (ROS), cytoplasmic Ca2+, mitochondrial membrane potential (MMP) and caspase-3 activity were analyzed using the flow cytometric assay. Apoptosis-associated proteins, such as p53, BAX, BCL-2, cytochrome c, caspase-3 and -9, EndoG and AIF were determined by Western blotting. Results: Our results showed that baicalein promoted the levels of p53, BAX, cytochrome c, capase-3 and -9 and reduced the level of BCL-2, which were associated with the induction of apoptotic cell death of SCC-4 cells. A release of cytochrome c from mitochondria into cytosol was demonstrated and an activation of caspase-3, which led to the occurrence of apoptosis in SCC-4 cells treated with baicalein as determined by Western blot. In order to understand the role of Ca2+ in the induction of apoptosis, cells were pre-treated with BAPTA (intracellular calcium chelator) and baicalein. It was shown that the MMP was restored, and the level of cytoplasmic Ca2+ suppressed, the proportion of cells undergoing apoptosis was also markedly diminished. Our data suggest that cellular Ca2+ modulates baicalein-induced cell death via a Ca2+-dependent mitochondrial death pathway in SCC-4 cells.
Relation:	IN VIVO (21 )1053 ~1058
Appears in Collections:	[Department of Biological Science and Technology] Journal articles

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