Effect of 5-methoxypsoralen(5-MOP) on cell apoptosis and cell cycle in human hepatocellular carcinoma cell line

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Title:	Effect of 5-methoxypsoralen(5-MOP) on cell apoptosis and cell cycle in human hepatocellular carcinoma cell line
Authors:	(Y.M.Lee)、(T.H.Wu)、(S.F.Chen)、鍾景光(Jing-Gung Chung)*
Contributors:	生命科學院生物科技學系;中國附醫醫學研究部
Keywords:	5-Methoxypsoralen (5-MOP);Human hepatocellular carcinoma cell line;Cell cycle;Apoptosis
Date:	2003.01
Issue Date:	2009-08-25 14:39:43 (UTC+8)
Abstract:	The chemopreventive role of 5-methoxypsoralen (5-MOP) in the human hepatocellular carcinoma (HCC) cell line was investigated by studying the regulation of proliferation and apoptosis in HCC (J5) cells. Morphological analysis, cell viability assay, DNA analysis and cell-cycle analysis suggest that there are at least three modes of the suppressive effects shown by 5-MOP: (a) kills J5 cells directly; (b) induces apoptosis by arresting J5 cells at the G2/M phase in the cell cycle; (c) induces apoptosis through an independent pathway with cell-cycle arrest at 24–72 h of exposure. Further immunoblot analysis demonstrated that inhibition of cyclin B1 by 5-MOP may play an important role in G2/M arrest of J5 cells and provides an additional way to prevent cells from entering the M phase and undergoing apoptosis. 5-MOP therefore appears to exert its anticarcinogenic properties by cytotoxic effect, inducing apoptosis and inhibiting proliferation in the human hepatocellular carcinoma cell line.
Relation:	TOXICOLOGY IN VITRO 17(3 )279 ~287
Appears in Collections:	[Department of Biological Science and Technology] Journal articles

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