Aloe-emodin induced in vitro G2/M arrest of cell cycle in human promyelocytic leukemia HL-60 cells

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Title:	Aloe-emodin induced in vitro G2/M arrest of cell cycle in human promyelocytic leukemia HL-60 cells
Authors:	(H.C. Chen)、謝文聰(Hsieh Wen-Tsong)、張文正、鍾景光(Jing-Gung Chung)*
Contributors:	生命科學院生物科技學系;中國附醫醫學研究部
Keywords:	Aloe-emodin;Human leukemia HL-60 cells;Apoptosis;Flow cytometric assay
Date:	2004.09
Issue Date:	2009-08-25 14:39:37 (UTC+8)
Abstract:	In this study,we have evaluated the chemopreventive role of aloe-emodin in human promyelocytic leukemia HL-60 cells in vitro by studying the regulation of proliferation,cell cycle and apoptosis. Aloe-emodin inhibited cell proliferation and induced G2/M arrest and apoptosis in HL-60 cells. Investigation of the levels of cyclins B1,E and A by immunoblot analysis showed that cyclin E level was unaffected,whereas cyclin B1 and A levels increased with aloe-emodin in HL-60 cells. Investigation of the levels of cyclindependent kinases,Cdk1 and 2,showed increased levels of Cdk1 but the levels of Cdk2 were not effected with aloe-emodin in HL-60 cells. The levels of p27 were increased after HL-60 cells were cotreated with various concentrations of aloe-emodin. The increase of the levels of p27 may be the major factor for aloe-emodin to cause G2/M arrest in these examined cells. Flow cytometric assays and DNA fragmentation gel electrophoresis also confirmed aloe-emodin induced apoptosis in HL-60 cells. The levels of caspase-3 were increased after HL-60 cells were cotreated with 10 lM aloe-emodin for 12,24, 48,and 72 hours. Taken together, aloe-emodin therefore appears to exert its anticarcinogenesis properties by inhibiting proliferation and inducing cell cycle arrest and apoptosis underwent activation of caspase-3 in human leukemia HL-60 cells.
Relation:	FOOD AND CHEMICAL TOXICOLOGY 42(8 )1251 ~1257
Appears in Collections:	[Department of Biological Science and Technology] Journal articles

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