The inhibitory effect of trilinolein on norepinephrine-induced β–myosin heavy chain promoter activity, reactive oxygen species generation, and extracellular signal-regulated kinase phosphorylation in neonatal rat cardiomyocytes.

CMUR > College of Life Sciences > Department of Biological Science and Technology > Journal articles > Item 310903500/6243

Please use this identifier to cite or link to this item: http://ir.cmu.edu.tw/ir/handle/310903500/6243

Title:	The inhibitory effect of trilinolein on norepinephrine-induced β–myosin heavy chain promoter activity, reactive oxygen species generation, and extracellular signal-regulated kinase phosphorylation in neonatal rat cardiomyocytes.
Authors:	(Liu JC);(Chan P);(Chen JJ);(Lee HM);(Lee WS);(Shih NL);(Chen YL);(Hong HJ);鄭志鴻(CHENG, TZU-HURNG)*
Contributors:	生命科學院生物科技學系
Keywords:	Norepinephrine;Trilinolein;Reactive oxygen species;Extracellular signal-regulated kinase;β-Myosin heavy chain gene
Date:	2004-01
Issue Date:	2009-08-25 14:39:18 (UTC+8)
Abstract:	The myocardial protective effects of trilinolein, isolated from the traditional Chinese herbSanchi (Panax notoginseng), are thought to be related to its antioxidant activity. However, the intracellular mechanism underlying the protective effect of trilinolein in the heart remains unclear. In the present study, we investigated the effect of trilinolein on norepinephrine (NE)-induced protein synthesis in cardiomyocytes. Cultured neonatal rat cardiomyocytes were stimulated with NE, then protein content, [3H]-leucine incorporation, and β-myosin heavy chain (β-MyHC) promoter activity were examined. The effect of trilinolein on NE-induced intracellular reactive oxygen species (ROS) generation was measured with a redoxsensitive fluorescent dye (2,7-dichlorofluorescin diacetate) and extracellular signal-regulated kinase (ERK) phosphorylation by Western blotting. Trilinolein inhibited NE-increased protein synthesis, β-MyHC promoter activity, and intracellular ROS. Both trilinolein and the antioxidant, N-acetyl-cysteine, decreased NE- and H2O2-induced protein synthesis, β-MyHC promoter activity, and ERK phosphorylation. These data indicate that trilinolein inhibits NE-induced protein synthesis via attenuation of ROS generation in cardiomyocytes.
Relation:	JOURNAL OF BIOMEDICAL SCIENCE 11(1)11 ~18
Appears in Collections:	[Department of Biological Science and Technology] Journal articles

Files in This Item:

File	Size	Format
	58Kb	Unknown	483	View/Open

Loading...