Ganoderma tsugae extracts inhibit colorectal cancer cell growth via G2/M cell cycle arrest

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 生命科學院 > 生物科技學系暨碩士班 > 期刊論文 > Item 310903500/6234

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题名:	Ganoderma tsugae extracts inhibit colorectal cancer cell growth via G2/M cell cycle arrest
作者:	(Shih-Chung Hsu)、(Chien-Chih Ou)、(Jhy-Wei Li)、(Tzu-Chao Chuang)、(Han-Pon Kuo)、(Jah-Yao Liu)、(Chin-Shiang Chen)、(Song-Chow Lin)、(Ching-Hua Su)、高銘欽(Ming-Ching Kao)
贡献者:	生命科學院生物科技學系
关键词:	Ganoderma tsugae;Lingzhi (Reishi);Colo205;Colorectal cancer;Cancer therapy
日期:	2008-10
上传时间:	2009-08-25 14:39:10 (UTC+8)
摘要:	Ethnopharmacological relevance Ganoderma, known as Lingzhi or Reishi, has been traditionally administered throughout Asia for centuries as a cancer treatment and for other medicinal purposes. Aim of the study To investigate the inhibitory activity and explore the molecular mechanisms of anti-tumor effect on colorectal cancer cells in vitro and in vivo as well as to test the side effects of Ganoderma tsugae. Materials and methods Methanol fraction was obtained from dried fruiting bodies of Ganoderma. TLC and HPLC were performed to differentiate and confirm the identification of different species as well as to quantify the bioactive molecules in methanol extracts of Ganoderma species. MTT and Trypan blue exclusion assay as well as tumorigenesis study were used to assess the anti-tumor effect in vitro and in vivo. Using flow cytometry and Western Blots, we examined further the molecular mechanisms of anti-tumor effect. Finally, biochemical and hematological profiles and pathological examinations were used to evaluate the safety. Results The Ganoderma tsugae extracts inhibit colorectal cancer cell proliferation caused by accumulating cells in G2/M phase, and it may be through downregulation of cyclin A and B1 and upregulation of p21 and p27. Tumorigenesis study in nude mice revealed the extracts caused tumor shrinkage. Additionally, safety assay showed Ganoderma tsugae extracts caused no significant side effects in an animal model. Conclusions This study provides molecular evidence that Ganoderma tsugae extracts exert anti-tumor effects both in vitro and in vivo on colorectal adenocarcinoma cells by inducing G2/M cell cycle arrest. More importantly, no significant physiological changes resulting from treatment with Ganoderma tsugae extracts were observed in the animal model. Therefore, these data provide new insights into the possible therapeutic use of Ganoderma tsugae for treating colorectal cancer.
關聯:	JOURNAL OF ETHNOPHARMACOLOGY 120(3)394~401
显示于类别:	[生物科技學系暨碩士班] 期刊論文

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