| 摘要: | Background and objective:
Albeit of heterogeneity of tissue-resident macrophage in origins which have been elicited in the early first decade of the 21 the century, other aspects of divergences about survival factors, activated processes, specific functions in distinctive tissues of these phagocytosis cells need to be explored. Since colony-stimulating factor 1 receptor (CSF1R) mediating signaling is the essential key for sustaining tissue macrophages, which has been reported in many patterns such microglia, Langerhans cells; we sought the simplified model to reveal the response of macrophages in many tissues under contingent on CSF1R by using PLX3397.
Method:
Six-week mice were fed by vehicle chow or PLX3397 290mg per kilogram vehicle chow for three time points: 3 days, 7 days and 21 days. Tissue macrophages were investigated by immunohistochemistry with markers IBA1, F4/80 in many organs: brain, lung, heart, liver, spleen, kidney, colon, adipose tissue, pancreas.
Results:
PLX3397 diverse the response of macrophages into two groups: partly depletion in some tissue in different locations and tested time points: brain, spleen, lung, liver, adipose tissue and dramatic reduction in macrophage population in heart, kidney, colon.
Conclusions:
Our results set up the whole landscape of macrophage in those tissues which require CSF1R signaling as the survival key to maintain the existence. Moreover, we reported the simple model with useful agent-PLX3397 to modulate the tissue macrophages which is great potential tool for macrophage-related models in suitable approach. |
