| 摘要: | 本研究目的為評估不同劑量杜仲萃取物對於代謝症候群模式大鼠改善阿茲海默症生化指標之效果。本實驗動物購入60隻雄性、六週齡的先天性高血壓老鼠（Spontaneous hypertensive rat, SHR）為實驗動物，餵食高脂飲食(佔30% 總熱量)連續八週，八週後以腹腔注射Streptozotocin（40 mg/kg B.W.溶於0.1M citrate buffer, pH4.3）連續兩天誘發第二型糖尿病，確立為糖尿病後進行實驗分組，將實驗動物隨機分為控制組(SHR)，阿茲海默症控制組(AD)及一倍、三倍、五倍杜仲萃取物組，並以Wistar Kyoto大鼠為正常對照(WKY)，於第0、4、8週採集血液檢體與進行非侵入式血壓測量，於實驗第九週至第十一週時進行十字迷宮試驗及物體辨認實驗，並於第十二週進行犧牲，採集血液、肝臟、腦部進行分析。當介入八週之後，有飲用杜仲萃取物之組別的大鼠，與控制組相較血壓均有顯著下降，且空腹血糖數值顯著低於阿茲海默症對照組(AD)；在肝臟脂質分析顯示，一倍劑量杜仲萃取物(AD＋D1)組可有效降低肝臟中三酸甘油酯及膽固醇酯的堆積；在腦部組織分析中顯示，一倍劑量杜仲萃取物(AD＋D1)組之海馬迴中類澱粉蛋白沉積有顯著下降；給予八週一倍劑量杜仲萃取物(133 mg/kg B.W.)可改善阿茲海默症的相關病程及生化指標之效果，未來亦可將杜仲萃取物做更廣泛的應用。
Alzheimer’s disease (AD) is a neurodegenerative disorder characterized by cognitive dysfunction. Metabolic syndrome is thought to play an important role on the progression of Alzheimer’s disease. The aim of the study was to evaluate the effect of Eucommia ulmoides extracts on Alzheimer’s disease biomarkers in animal model of metabolic syndrome. Six-week-old, male, spontaneously hypertensive (SHR, n=50) and Wistar Kyoto(abbreviated WKY, n=10) rats were divided into six group (n=10): Normal group (WKY rats); Control group (SHR); AD group (SHR rats, injected with 40 mg/kg B.W. of Streptozotocin); 133、399、665 mg/kg of Eucommia ulmoides extracts in AD rats (AD＋D1、AD＋D3、AD＋D5), and all the rats were fed with high-fat diet to induce the model of metabolic syndrome. During the study period, blood glucose, insulin, blood pressure, and lipid profile were collected. After eight weeks of different doses of Eucommia ulmoides extracts treatment, the results showed that all the groups fed with Eucommia ulmoides extracts can significantly lower the level of blood pressure and fasting blood glucose, compared to the control group. Compared to the AD group, the level of hepatic triglycerides and hepatic cholesterol were significantly decreased in AD＋D1 group. The result also showed that the beta-amyloid in hippocampus, which was the main pathologic marker in AD was significantly decreased in AD＋D1 group. In conclusion, the group fed with 133 mg/kg B.W. of Eucommia ulmoides extracts can improve the metabolic syndrome and the Alzheimer’s disease-like biomarkers. Furthermore, Eucommia ulmoides extracts can be more applicable to the functional food in the future. |
