The proliferation and migration effects of huangqi on RSC96 Schwann cells

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Title:	The proliferation and migration effects of huangqi on RSC96 Schwann cells
Authors:	(Wen-Kuei Fang);(Fu-Yang Ko);(Hwai Lee Wang);呂明進(Ming-Chin Lu);(Li-Ming Chen);蔡輔仁(Fuu-Jen Tsai);蔡長海(Chang-Hai Tsai);陳悅生(Yueh-Sheng Chen);郭薇雯(Wei-Wen Kuo);黃志揚(Chih-Yang Huang)
Contributors:	中醫學院學士後中醫學系學士班中醫診斷學科
Keywords:	Huangqi;Proliferative Effect;Schwann Cells;Sciatic Nerve;Peripheral Nerve Regeneration
Date:	2009
Issue Date:	2009-08-24 15:02:41 (UTC+8)
Abstract:	This study evaluated the proliferation effects of huangqi on neuron regeneration. We investigated the molecular mechanisms, which include: (1) cyclin D1, A, E-cell cycle factors and MAPK signaling proliferation (2) FGF-2-UPA-MMPs migration signaling. After treatment with various Huanqi concentrations (1.25, 12.5, 125, 250 and 500 microg/ml,), we observed that Huanqi can increase Rsc 96 cell proliferation at 12.5 microg/ml (p < 0.01) concentration determined by the MTT and wound healing tests. Examination by RT-PCR and Western blotting assay showed that Huangqi is able to stimulate the mRNA and protein expressions of cyclin D1, A, E, cell cycle controlling proteins and excite ERK and P38 MAPK signaling pathways to promote cell proliferation. Huangqi stimulates the FGF-2-UPA-MMP 9 migration pathway and enhances RSC 96 Schwann cells migration. Using MAPK chemical inhibitors, U0126, SB203580 and SP600125, the proliferative effects of Huangqi on RSC 96 cells were ERK and P38 signaling-dependent. Based on these results, applying an appropriate dose of Huangqi with biomedical materials would be a potential approach to enhancing neuron regeneration.
Relation:	AMERICAN JOURNAL OF CHINESE MEDICINE ( ) ~
Appears in Collections:	[Graduate Institute of Acupuncture Science] Journal articles

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