Effector Mechanisms of Norcantharidin-Induced Mitotic Arrest and Apoptosis in Human Hepatoma Cells.

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題名:	Effector Mechanisms of Norcantharidin-Induced Mitotic Arrest and Apoptosis in Human Hepatoma Cells.
作者:	(Yan-Nian Chen);陳榮洲(Jung-Chou Chen);(Sui-Chu Yin);(Guang-Sheng Wang);(Wei Tsauer);許昇峰(Sheng-Feng Hsu);(Shih-Lan Hsu)*
貢獻者:	中醫學院學士後中醫學系學士班中醫內科學科
關鍵詞:	norcantharidin;cyclin B;Cdc25c;apoptosis;caspase;Bcl-2
日期:	2002
上傳時間:	2009-08-24 15:02:00 (UTC+8)
摘要:	NCTD is a demethylated form of cantharidin with antitumor properties, which is now in use as a routine anticancer drug against hepatoma. However, there is limited information on the effect of NCTD on human cancer cells. In the present study, NCTD inhibited proliferation, caused mitotic arrest, then progressed to apoptosis within 96 hr in 3 human hepatoma cell lines: HepG2, Hep3B and Huh-7. NCTD treatment (5 g/ml) enhanced the expression of Cdc25C and p21Cip1/Waf1, increasing the phosphorylation of these 2 proteins. In addition, NCTD treatment induced an earlier increase in cyclin B1-associated histone H1 kinase activity within 48 hr, but an approximately 70% reduction of both protein level and kinase activity of cyclin B1 was observed at 72 hr. Treatment with NCTD significantly decreased the expression of p53 protein but did not affect the expression of Cdk1 and p27Kip1. Moreover, NCTD treatment also increased the phosphorylation of Bcl-2 and Bcl-XL but did not affect the expression of Bax or Bad. Bcl-2 phosphorylation appears to inhibit its binding to Bax since less Bax was detected in immunocomplex with Bcl-2 in NCTD-treated HepG2 cells. In addition, NCTD treatment caused activation of caspase-9 and caspase-3, preceding DNA fragmentation and morphologic features of apoptosis. Pretreatment with the broad-spectrum caspase inhibitor z-VAD-fmk markedly inhibited NCTD-induced caspase-3 activity and cell death. These results suggest that phosphorylation of p21Cip1/Waf1 and Cdc25C and biphasic regulation of cyclin B1-associated kinase activity may contribute to NCTD-induced M-phase cell-cycle arrest. Furthermore, the increase of p21Cip1/Waf1, phosphorylation of Bcl-2 and Bcl-XL, activation of caspase-9 and caspase-3 may be the molecular mechanism through which NCTD induces apoptosis.
關聯:	INTERNATIONAL JOURNAL OF CANCER 100(2)158 ~165
顯示於類別:	[針灸研究所] 期刊論文

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