Sodium ascorbate inhibits growth via the induction of cell cycle arrest and apoptosis in human malignant melanoma A375.S2 cells.

CMUR > College of Chinese Medicine > Graduate Institute of Acupuncture Science > Journal articles > Item 310903500/5669

Please use this identifier to cite or link to this item: http://ir.cmu.edu.tw/ir/handle/310903500/5669

Title:	Sodium ascorbate inhibits growth via the induction of cell cycle arrest and apoptosis in human malignant melanoma A375.S2 cells.
Authors:	(Lin SY)ã(Lai WW)ã(Chou CC)ã(Kuo HM)ãæå¾·è(Li TM)ãé¾æ¯å(Jing-Gung Chung)ã(Yang JH)
Contributors:	ä¸é«å¸é¢éç¸ç ç©¶æ
Date:	2006.11
Issue Date:	2009-08-24 14:59:50 (UTC+8)
Abstract:	Vitamin C has been reported to be useful in the treatment and prevention of cancer. Inconsistent effects from growth stimulation to induction of apoptosis of malignant tumor cells, however, have been reported. Melanoma is an increasingly common and potentially lethal malignancy. It was reported that melanoma cells were more susceptible to ascorbate toxicity than any other tumor cells. The mechanisms accounting for ascorbate-induced apoptosis in human melanoma cells, however, have remained unclear. This study was undertaken to investigate the effect of sodium ascorbate on cytotoxicity and apoptosis in human malignant melanoma A375.S2 cells. A375.S2 cells were incubated with a certain range of concentrations of sodium ascorbate for various time periods. In order to examine the effects of sodium ascorbate on cell proliferation, cell cycle, apoptosis and necrosis, we performed 4,6-diamidino-2-phenylindole dihydrochloride assays and flow cytometry analysis. Polymerase chain reaction was used to examine the mRNA levels of p53, p21, p27, cyclin A, cyclin E, CDK2 and CDK4, which are associated with cell cycle S-phase arrest and apoptosis. Flow cytometric analysis showed that sodium ascorbate significantly induced cell cycle arrest and apoptosis in the A375.S2 cell line in a dose-dependent manner. The increased expressions of p53 and p21, and the decreased expressions of cyclin A, cyclin E, CDK2 and CDK4, indicated the cell cycle arrest at G1/S phase after the cells had been treated with sodium ascorbate. Induction of apoptosis involved an increase in the levels of p53, p21 and cellular Ca2+, and a decrease in mitochondrial membrane potential and activation of caspase 3 before culminating in apoptosis in sodium ascorbate-treated A375.S2 cells.
Relation:	MELANOMA RESEARCH 16(6 )509 ~519
Appears in Collections:	[Graduate Institute of Acupuncture Science] Journal articles

Files in This Item:

File	Description	Size	Format
開會.txt		2Kb	Text	426	View/Open

Loading...