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    題名: 魚腥草對肺癌細胞之活性及作用機轉研究;Bioactivities and action mechanisms of Hottuynia cordata on lung cancer cells
    作者: 張文鑫;Wen-Shin Chang
    貢獻者: 中國醫藥大學:基礎醫學研究所
    關鍵詞: 魚腥草;肺癌;生物鹼;細胞週期;凋亡;Hottuynia cordata;lung cancer;alkaloid;cell cycle;apoptosis
    日期: 2009-07-13
    上傳時間: 2009-08-11 14:35:51 (UTC+8)
    摘要: 肺癌於台灣居十大死亡癌症首位,除了現今的化學療法,中草藥之使用或許對肺癌有其參考價值。在台灣,魚腥草是一種很常用的中草藥,它被廣泛的應用在各類的疾病。回顧相關文獻記載:魚腥草具有許多作用,包括:抗發炎、抗氧化、抗病毒、免疫調節作用、抗急性呼吸窘迫症候群、抗菌和抗腫瘤活性。然而未見有文章陳述魚腥草對抗肺癌的作用,因此本研究目的在評估魚腥草抗肺癌之可能的作用與機轉。使用ICR品系的小鼠進行腳蹠浮腫實驗以評估魚腥草之抗發炎作用,並選用2株肺癌細胞A549 和LLC細胞,進行有關魚腥草對肺癌細胞的存活率,對肺癌細胞形態的改變,對細胞週期之影響,以及造成肺癌細胞凋亡的作用探討;並分析魚腥草對細胞週期及細胞凋亡相關蛋白質之影響,以評估魚腥草對肺癌細胞之作用及其可能之機轉。
    利用人類肺癌細胞株A549和小鼠肺癌細胞株LLC進行抗癌活性評估,結果發現魚腥草粗抽物具有抑制肺癌細胞活性之作用。由人類的肺癌細胞株 (A549) 之實驗結果得知:魚腥草的粗抽物會引起人類肺癌細胞 (A549) 細胞週期的停止,其作用可能是透過向下調節細胞週期中的G1期相關的蛋白質所致。又魚腥草含有數種生物鹼,以其中對A549細胞較具抑制效果的3種Aristolactam BII, Aristolactam AII 和 Noraristolodione進行作用之分析,研究發現這些生物鹼 ( Aristolactam BII, Aristolactam AII 和 Noraristolodione )會使A549肺癌細胞之細胞週期在S或 G2/M期產生蓄積作用,使相關的蛋白質向下調節。由本實驗結果可知,魚腥草粗萃取物及其所含生物鹼成分Aristolactam BII, Aristolactam AII 和 Noraristolodione對於人類非小細胞肺癌細胞株A549細胞的影響,主要是透過造成肺癌細胞週期生長停滯及誘導凋亡。

    Lung cancer is the leading cause among the top ten-cause of death in Taiwan. Besides the currently used chemotherapy, herbal medicine may play a role in the treatment of lung cancer. Hottuynia cordata THUNB (Saururaceae; HC), one of the frequently used herbal medicine in Taiwan, has been widely used in various diseases. Review from literatures, HC had many effects, including anti-inflammatory, anti-oxidative, anti-viral, immunomodulatory, anti-SARS, anti-bacterial and anti-tumor activities. However, there is no literatures describe its effect on lung cancer. The aim of my present study is to evaluate the possible effect and action mechanism of HC on lung cancer. A549 and LLC lung cancer cell lines were used to evaluate the effects of HC on the cell viability and possible anti-tumor effects,and SD rats were used in anti-inflammation study.
    The methods of experiment, including paw edema, cell viability, cell morphology, cell cycle, cell apoptosis and protein expression.
    We used A549 and LLC cells in the evolution of anticancer activity, and found that HC-crude extract showed inhibitory effect on cell viability of A549 and LLC cells. In order to look into the possible potential and action mechanism of HC on human lung cancer, human lung cancer A549 cells were used. Results from our study, HC treatment causes cell cycle arrest of A549 cells. The inhibition of cell cycle might be via the down-regulating of G1 phase related protein levels such as cyclinD1, cyclinE, cyclinA, CDK4, CDK6, and CDK2. Additionally, HC causes A549 cell apoptosis via caspase-8 and P27 expression. The anti-tumor effect of HC-crude extract and active compounds (aristolactam BII, aristolactam AII and noraristolodione ) on human lung carcinoma A549 cell line is via cell cycle arrest and late apoptosis.
    顯示於類別:[基礎醫學研究所] 博碩士論文

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