樟芝三萜類成分抗血癌之相關藥理計算分析研究

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題名:	樟芝三萜類成分抗血癌之相關藥理計算分析研究 Study of computational analysis of Antrodia camphorate triterpenoid’s anti-leukemia pharmacology
作者:	劉祐瑄;Yu-Hsuan Liu
貢獻者:	生物科技學系碩士班
關鍵詞:	樟芝;三萜血癌;抗血癌;急性早幼粒細胞白血病;Antrodia camphorate;triterpenoids;leukemia;anti-leukemia;Acute promyelocytic leukemia
日期:	2014-06-10
上傳時間:	2014-10-02 09:40:50 (UTC+8)
出版者:	中國醫藥大學
摘要:	樟芝是中國傳統民間用藥之一，常用於抗發炎、治療皮膚搔癢、解酒精中毒等，有研究指出，它有許多成分: 多糖、三萜、腺苷等(1)，其中，三萜類具有抗血癌、抗乳腺癌、抗肺癌等(2)功效。急性早幼粒細胞白血病(acute promyelocytic leukemia,APL)是因15(q22)和17(q11–12)染色體互相移位造成，使PML, RARα基因融合，讓早幼粒細胞異常增殖和積聚在骨髓及血液中，且致死率高(3)。有研究指出樟芝的Zhankuic acid A三萜，與TRAIL(TNF-related apoptosis-inducing ligand)結合，使DR5(Death receptor 5)表現，造成NFκB活化、Bax/Bcl-2表現上升，使HL-60細胞凋亡(4)。本研究比較不同藥物三萜，如:樟芝、人参、甘草、柴胡、茯苓，加上市售抗血癌藥物，利用Discovery Studio○R計算分析，分析不同藥物三萜及抗血癌藥物與腫瘤壞死因子（TNF）受體相關死亡結構域蛋白（TRADD）的對接情形與對接分數，探討樟芝三萜是否為最主要抗血癌成分。初步結果顯示:樟芝三萜結構及抗血癌藥物，都與蛋白接合。樟芝三萜結構較基本，分子較小，上面含一些OH基及氫原子，較易有氫鍵鍵節與蛋白接合，所以，接合較穩固，對接分數也較高。 Antrodia camphorate(AC) is one of the traditional Chinese folk medicine, often used in anti-inflammatory, to treat skin itching, solution alcoholism, etc.Some reports have suggested that it has many ingredients: polysaccharides, triterpenes, adenosine(1),triterpenoids with anti-leukemia, breast cancer, lung cancer (2) effect. Acute promyelocytic leukemia (APL) is the result of reciprocal translocation between chromosomes 15(q22) and 17(q11–12),making the PML, RARα gene fusion, so promyelocytic abnormal proliferation and accumulation in the bone marrow and blood, and the mortality rate is very high(3).AC in Zhankuic acid A triterpene composition,through TNF-related apoptosis-inducing ligand(TRAIL) induced the expression of death receptor 5(DR5), the DR5 expression,resulting in NFκB activation, Bax/Bcl-2 increase expression, induced HL-60 cells apoptosis(4). This study compared numbers of different medicinal triterpenoids, such as: Antrodia, Ginseng, Licorice, Bupleurum, Poria and anti-leukemia medicine, using the Discovery Studio○Rcomputational analysis, different kinds of medicines triterpenoids and anti-leukemia medicine analysis and Tumor necrosis factor (TNF)-receptor 1-associated death domain protein (TRADD) docking situations and docking scores, and explored whether AC triterpenoids is the most important ingredient in its anti-leukemia effect. Preliminary results showed that AC triterpenoid structure and anti-leukemia medinice are bonding with protein. AC triterpenoid structure is relatively basic, small molecules, and also some OH group and the hydrogen atoms, more to have hydrogen bonding and protein bonding, therefore, the more firmly bonded docking score is higher.
顯示於類別:	[生物科技學系暨碩士班] 博碩士論文

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