針灸對kainic acid治療大鼠癲癇病灶形成效用之研究; The effect of acupuncture on the development of epileptic focus formation in kainic acid-treated rat

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题名:	針灸對kainic acid治療大鼠癲癇病灶形成效用之研究;The effect of acupuncture on the development of epileptic focus formation in kainic acid-treated rat
作者:	陳威良;Wei-Liang Chen
贡献者:	中國醫藥大學：針灸研究所
关键词:	手捻針;電針;kainic acid;Mossy fiber sprouting;Astrogliosis;Manual acupuncture;Electroacupunctur
日期:	2008-06-13
上传时间:	2009-08-11 14:24:07 (UTC+8)
摘要:	用針灸來治療癲癇在中國已有很久的歷史，但根據我們所知，尚未有用針灸來預防癲癇病灶形成的報告。因此本研究的目的是觀察針灸對於預防癲癇病灶形成的效用。我們將kainic acid（12 mg/kg, KA）於Spragrue-Dawley（SD）大鼠的腹腔注射，六星期後建立一個慢性癲癇模式。KA注射一星期後將大鼠隨機分組，並於相當人類的百會（GV20）和風府（GV16）穴處分別於施予手捻針（manual acupuncture, MA）、2 Hz電針（electroacupuncture, EA）和100 Hz電針刺激。KA注射後六星期將大鼠犧牲取腦，並做成病理切片。用Timm’s staining觀察mossy fiber sprouting，NeuN免疫化學組織染色觀察神經細胞（neuron），用glial fibrillary acidic protein（GFAP）免疫化學組織染色觀察astrogliosis。結果顯示MA、2 Hz EA和100 Hz EA都能減少海馬區域齒狀回(dentate gyrus) Timm’s staining的optical density difference；2 Hz EA和100 Hz EA能減少海馬CA1區域的NeuN免疫反應細胞；MA和100 Hz EA減少海馬hilus區域的GFAP免疫反應細胞；2 Hz EA和100 Hz EA能減少海馬striatum oriens和striatum radiate區域GFAP免疫反應細胞。我們的結果顯示MA、2 Hz電針和100 Hz電針在百會和風府穴治療五星期能夠減少SD大鼠KA注射後六星期的mossy fiber sprouting和astrogliosis，推測 MA、2 Hz電針和100 Hz電針能預防癲癇病灶的形成發展（the development of epileptic focus formation），即有anti-epileptogenesis的作用。2 Hz電針能夠減少neuron的喪失，是否2 Hz電針對於保護神經細胞有較大的作，需要更進一步的研究。 Acupuncture has been used for epilepsy treatment for a long period of time in China. However, to our knowledge, the efficacy of acupuncture for preventing the development of epileptic focus formation is not yet elucidated until now. Therefore, the purpose of the present study was to investigate the effect of acupuncture on the development of epileptic focus formation. We establish a chronic epileptic model by using intraperitoneal administration of kainic acid (12 mg/kg, KA) in Spragrue-Dawley (SD) rats for 6 weeks. The rats were randomly divided into groups in which they receive the manual acupuncture (MA), 2 Hz electroacupuncture (EA), 100 Hz EA on Baihui acupoint (GV20) and Fengfu acupoint (GV16), respectively, one week after KA administration. The rats were sacrificed 6 weeks after KA administration and the brain tissue was removed for section. The Timm’s staining was done for mossy fiber sprouting, NeuN staining for neuron, and glial fibrillary acidic protein (GFAP) staining for astrogliosis. The results indicated that MA, 2 Hz EA and 100 Hz EA reduced optical density difference of Timm’s staining of hippocampal dentate gyrus; 2 Hz EA and 100 Hz EA reduced NeuN immnuoreactive cells in the CA1 regions of hippocampus; MA and 100 Hz EA reduced GFAP immunoreactive cells in the hilus region of hippocamous, and 2 Hz EA and 100 Hz EA reduced GFAP immunoreactive cells in the striatum oriens and stratum radiate regions of hippocampus. In conclusion, our results indicated that MA, 2 Hz EA and 100 Hz EA at GV20 and GV16 for 5 weeks can reduce mossy fiber sprouting and also can reduce astrogliosis 6 weeks after KA administration in SD rats, suggesting that MA, 2 Hz EA and 100 Hz EA may prevent the development of epileptic focus formation, i.e. anti-epileptogenesis. In addition, 2 Hz EA may reduce neuron loss of hippocampus, whether or not 2 Hz may produce a greater effect in protection of neuron, further study is needed.
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