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| 題名: | 2007-2011年柯沙奇A9病毒之流行病學分析
Analysis of coxsackievirus A9 isolated from 2007 to 2011 |
| 作者: | 林怡婷;I-Ting Lin |
| 貢獻者: | 醫學檢驗生物技術學系碩士班 |
| 關鍵詞: | 科沙奇A9病毒;coxsackievirus A9 |
| 日期: | 2013-08-27 |
| 上傳時間: | 2013-10-02 11:28:13 (UTC+8) |
| 出版者: | 中國醫藥大學 |
| 摘要: | 腸病毒屬於小RNA 病毒科，其好發於春夏，嬰幼兒較易被其感染。本研究分析2007-2011 年間柯沙奇A9 病毒之流行趨勢發現，2011 年柯沙奇A9 病毒之陽性率(1.35%)較高於2007－2010 年間(其陽性率分別為: 0.04%， 0.03%， 0.04% 和 0.03%)。為了解陽性率驟升之因，首先利用統計學方法比較其檢體來源、好發年齡、發生季節與之前是否有所差異。統計發現2007 至2010 年間培養出柯沙奇A9 病毒檢體來源大部分為喉嚨拭子(90%)；好發年齡多為五歲以下幼童(90%)；而發生季節則無明顯差別(春秋兩季僅佔30%)。而2011 年之檢體來源
喉嚨拭子仍為多數(93.6%)；好發年齡五歲以下幼童約為59.2%，10歲以下幼童約20%，青壯年(11-50 歲)增為20.8%；發生於春夏兩季則佔96%。腸病毒如同其他RNA 病毒,在其基因複製時，因為缺乏自我
修正的能力(proofreading activity)而有高突變率發生，故懷疑此次之高陽性率為病毒序列改變造成。此研究利用One-step RT-PCR 取得VP1
全長(約900b.p；nt2440~3331)並分析其序列變化。由之前相關研究發現科沙奇A9 病毒可分為13 種Genotype。此次分析發現2007 至2011年之科沙奇A9 病毒株均與2005 年中國大陸爆發之科沙奇A9 病毒同
為Genotype XIII。
Coxsackievirus A9 is a member of human enterovirus genus in the picornaviridae family, infecting children and infants in spring and summer seasons. The study investigated the prevalence and molecular epidemiology of Coxsackievirus A9 between 2007 and 2011 in Taiwan. The prevalence peak of Coxsackievirus A9 occurred in 2011. The positive rate of Coxsackievirus A9 was 13.5% in 2011, as higher than 0.04% in 2007, 0.03% in 2008, 0.04% in 2009, and 0.03% in 2010, respectively. Clinical features of infected cases during this five-year period indicated throat swabs were the main specimens for the positive isolation, as approximately 90%; children under the age of five were the major cases (90%) among indentified patients. However, no difference in epidemic seasons was observed; 30% was isolated in spring and fall, respectively. In the peak year of 2011, 93.6% among cases was identified from throat swabs. Age distribution revealed children under the age of five as approximately 59.2%, under ten as approximately 20%, and adults as 20.8%. The epidemic seasons occurred in spring and summer that was 96% among the prevalence in 2011. For analyzing the molecular epidemiology, VP1 genes (891 bp, nucleotides 2440~3331) from isolates during the five-year period were amplified using one-step RT-PCR, then directly sequenced. Phylogenetic tree analyzed Coxsackievirus A9 isolates between 2007 and 2011 as Genotype XIII, exhibiting the same genotype of Coxsackievirus A9 outbreak in Mainland China in 2005. |
| 顯示於類別: | [醫學檢驗生物技術學系暨碩士班 ] 博碩士論文
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