| 摘要: | 本研究目的為探討金皇石斛與黃花石斛萃取的石斛多醣，對結腸癌前期病變及結腸癌的影響。
實驗一，由氧化偶氮甲烷誘發的小鼠結腸癌前病變，腹腔注射，一星期一次，總共兩星期，口服投予DTTPS與DTPS為期六週的治療過程。DTTPS與DTPS顯著性的減少異常腺窩灶與異常腺窩的數目，在反轉錄聚合?○s鎖反應分析結果顯示，口服投予DTTPS與DTPS能顯著降低aldose reductase、 glutathione reductase、heme oxygenase-1、iNOS及COX-2 mRNA的表現量。西方墨點法分析結果顯示，口服投予DTTPS與DTPS能顯著降低增生細胞核抗原(proliferating cell nuclear antigen; PCNA)、以及NF-E2 related factor-2 (Nrf2)、iNOS與COX-2 蛋白質的表現量。DTTPS與DTPS能經由抗氧化及抗發炎作用預防結腸癌發生。
實驗二，小鼠結腸癌病變之誘發是腹腔注射氧化偶氮甲烷，一星期一次，總共六星期，同時口服投予DTTPS與DTPS為期二十四週。DTTPS與DTPS顯著性的減少異常腺窩灶與異常腺窩的數目，反轉錄聚合?○s鎖反應分析結果顯示，口服投予DTTPS與DTPS能顯著降低insulin-like growth factor 1 receptor (IGF-IR)、phosphase-glycogen synthase kinase 3 beta (pGSK3β )、pAkt-1和β-catenin mRNA的表現量。這些結果顯示DTTPS與DTPS能夠抑制氧化偶氮甲烷誘發的結腸癌。
結論，本研究顯示，DTTPS與DTPS具有腸道保健之效益，有助於結腸癌的防治。DTTPS的抑制結腸癌效果大於DTPS。
This study investigated the effect of Dendrobium Taiseed Tosnobile extracts (DTTPS) and Dendrobium tosaense extracts (DTPS) on preneoplastic lesions of colon and colon cancer in mice.
Experiment 1, preneoplastic lesions of colon in mice were induced by intraperitoneal injection of azoxymethane (AOM), once in a week for two weeks. AOM injected mice were orally treated with DTTPS or DTPS for entire period, 6 weeks. DTTPS or DTPS caused a significant decrease in the number of aberrant crypt foci (ACF) and aberrant crypt (AC). RT-PCR analysis showed that the colonic mucosa of AOM-treated mice expressed high levels of mRNA of aldose reductase, glutathione reductase, heme oxygenase-1, iNOS and COX-2, and DTTPS or DTPS caused a marked decrease in the expression of these mRNA. Western blot analysis showed that the colonic mucosa of AOM-treated mice expressed high levels of protein of proliferating cell nuclear antigen, NF-E2 related factor-2, iNOS and COX-2, and DTTPS or DTPS caused a marked decrease in the expression of these proteins. These results indicated that DTTPS or DTPS might exert colon cancer preventive activities through antioxidation and anti-inflammation.
Experiment 2, colon cancer in mice were induced by intraperitoneal injection of AOM, once in a week for six weeks. AOM injected mice were orally treated with DTTPS or DTPS for entire period, 24 weeks. DTTPS or DTPS caused a significant decrease in the number of ACF and AC. RT-PCR analysis showed that the colonic mucosa of AOM-treated mice expressed high levels of mRNA of insulin-like growth factor 1 receptor (IGF-IR), phosphase-glycogen synthase kinase 3 beta (pGSK3β ), pAkt-1 and ??-catenin, and DTTPS or DTPS caused a marked decrease in the expression of these mRNA. These results indicated that DTTPS or DTPS might exert inhibiting AOM-induced colon cancer.
In conclusion, the present study demonstrated that both DTTPS and DTPS can act as an effective chemopreventive agents against colon cancer, and the potency of DTTPS was larger than DTPS. |
