足三里穴位注射第一型瞬態電位類香草受器(TRPV1)促效劑而非第四型瞬態電位類香草受器(TRPV4)與第三型酸敏感離子通道(ASIC3) 促效劑能在小鼠產生類針灸止痛效應:第一型瞬態電位類香草受器可能是針灸刺激的感應通道?

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 中醫學院 > 針灸研究所 > 博碩士論文 > Item 310903500/50266

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题名:	足三里穴位注射第一型瞬態電位類香草受器(TRPV1)促效劑而非第四型瞬態電位類香草受器(TRPV4)與第三型酸敏感離子通道(ASIC3) 促效劑能在小鼠產生類針灸止痛效應:第一型瞬態電位類香草受器可能是針灸刺激的感應通道? TRPV1 but not TRPV4 and ASIC3 agonist injection replicates acupuncture-like analgesic effect at Zusanli acupoint (ST36) in mice: Possible role of mechanosensitive TRPV1 as “acupuncture responding channel”?
作者:	吳書毅;Shu-Yih Wu
贡献者:	針灸研究所碩士班
关键词:	第一型瞬態電位類香草受器;第四型瞬態電位類香草受器;第三型酸敏感離子通道;針灸;力學傳遞;TRPV1;TRPV4;ASIC3;Acupuncture;Mechanotransduction
日期:	2013-06-29
上传时间:	2013-10-02 11:04:41 (UTC+8)
出版者:	中國醫藥大學
摘要:	針灸是一種在穴位做力學刺激的治療。已經有許多的研究指出針灸機轉是藉由鴉片類胜?(opioid peptide)與腺嘌呤核?(adenosine)進行神經調控，但儘管如此我們仍對於物理性刺激如何變成神經訊號有許多不解。第一型和第四型瞬態電位類香草受器(TRPV1與TRPV4)與第三型酸敏感離子通道(ASIC3)已被認定可能是機械式感應通道(mechanosensitive channel)。這篇論文的目的在於探討，這三個通道在足三里穴位的角色。首先，從足三里與偽穴位(sham point)的組織採樣中發現，這三個通道在足三里有較多的表現。第二，從免疫螢光染色發現這三個通道在神經細胞與非神經細胞皆有表現。第三，將這三個通道的促效劑(agonist)注射到有發炎性疼痛小鼠的足三里內，發現只有TRPV1的促效劑(辣椒素，capsaicin)可以產生跟針灸一般的止痛效果。由於機械力感應已經被證實可以藉由鈣離子波(calcium wave propagation)將訊號由非神經細胞傳遞到神經纖維。因此，針灸的感傳也許包含了神經細胞發起與非神經細胞發起的兩種感知途徑。而鈣離子波的相關蛋白皆與TRPV1表現在相同的解剖層的結果也支持了上述想法。這篇論文的結果可以使我們更清楚針灸的感應介面，包含物理性刺激如何被接受而又轉換為生物訊號。 Acupuncture is a therapy that involves applying mechanical stimulation to acupoints using needles. Although many authors have reported acupuncture analgesia related to neural regulation by opioids or adenosine, little is known about how physical stimulation is turned into neurological signaling. The transient receptor potential vanilloid receptors 1 and 4 (TRPV1 and TRPV4) and the acid-sensing ion channel 3 (ASIC3) are regarded as mechanosensitive channels. This thesis aimed to clarify their roles at the Zusanli acupoint (ST36). First, tissues from ST36 and the sham point were collected. Then an abundance of channels in ST36 were demonstrated. Second, the immunofluorescence microscopic slides showed the channels were expressed in neural and non-neural cells. Third, agonists of channels were injected into ST36 and tested in a mouse inflammatory pain model. Only capsaicin, a TRPV1 agonist, replicated the analgesic effect of acupuncture. Mechanical sensing was reported by non-neural cells and processed to nerve terminals via calcium wave propagation (CWP). It is likely that both neural and non-neural cell initiated sensing pathways are triggered during acupuncture. This was supported by co-expression of TRPV1 and CWP components in the same anatomical layers. The results may lead to a better understanding of the interface between physical stimulation by acupuncture and biological signaling to neurons.
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