Aloe-emodin inhibited N-acetylation and DNA adduct of 2-aminofluorene and arylamine N-acetyltransferase gene expression in mouse leukemia L 1210 cells.

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Title:	Aloe-emodin inhibited N-acetylation and DNA adduct of 2-aminofluorene and arylamine N-acetyltransferase gene expression in mouse leukemia L 1210 cells.
Authors:	鍾景光(Jing-Gung Chung)*;(Yu-Ching Li);(Yi-Min Lee);林景彬(Jing-Pin Lin);(Kwork-Chui Chang);張文正
Contributors:	醫學院生物科技學系;中國附醫醫學研究部
Keywords:	N-Acetyltransferase;Aloe-emodin;Mouse leukemia cells;Arylamines (N-acetyl-2-aminofluorene and 2-aminofluorene)
Date:	2003-01
Issue Date:	2009-08-21 11:03:46 (UTC+8)
Abstract:	N-Acetyltransferases (NATs) plays an important role in the first step of arylamine compounds metabolism. Polymorphic NAT is coded for rapid or slow acetylatoion phenotypes, which are recognized to affect cancer risk related to environmental exposure. Aloe-emodin has been shown to exit anticancer activity. The purpose of this study is to examine whether or not aloe-emodin could affect arylamine N-acetyltransferase (NAT) activity and gene expression (NAT mRNA) and DNA–2-aminofluorene (DNA–AF) adduct formation in mouse leukemia cells (L 1210). By using high performance liquid chromatography, N-acetylation and non-N-acetylation of AF were determined and quantitated. By using reverse transcriptase-polymerase chain reaction (RT-PCR) and PCR,NATmRNAwas determined and quantitated. Aloe-emodin displayed a dose-dependent inhibition to cytosolic NAT activity and intact mice leukemia cells. Time-course experiments indicated that N-acetylation of AF measured from intact mice leukemia cells were inhibited by aloe-emodin for up to 24 h. Using standard steady-state kinetic analysis, it was demonstrated that aloe-emodin was a possible uncompetitive inhibitor to NAT activity in cytosols. The DNA–AF adduct formation in mouse leukemia cells were inhibited by aloe-emodin. The NAT1 mRNA in mouse leukemia cells were also inhibited by aloe-emodin. This report is the first demonstration which showed aloe-emodin affect mice leukemia cells NAT activity, gene expression (NAT1 mRNA) and DNA–AF on adduct formation.
Relation:	LEUKEMIA RESEARCH 27(9):831~840
Appears in Collections:	[Department of Biological Science and Technology] Journal articles

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