槴子？與其代謝產物抗幽門螺旋桿菌引發胃癌之體內與體外效能評估

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 生命科學院 > 生物科技學系暨碩士班 > 研究計畫 > Item 310903500/47684

請使用永久網址來引用或連結此文件: http://ir.cmu.edu.tw/ir/handle/310903500/47684

題名:	槴子？與其代謝產物抗幽門螺旋桿菌引發胃癌之體內與體外效能評估 The Effects of Geniposide and Its Metabolites on Anti-Helicobacter Pylori Infection in Vitro and in Vivo
作者:	鍾景光(Jing-Gung Chung)
貢獻者:	生命科學院生物科技學系;中國附醫醫學研究部
關鍵詞:	梔子?；梔子素；幽門螺旋桿菌；抗發炎;geniposide;genipin;Helicobacter pylori;antiinflammation
日期:	2013-07-30
上傳時間:	2013-01-25 13:57:45 (UTC+8)
摘要:	梔子常用來治療肝炎與黃疸等發炎及發熱等症狀。已經證實之活性包括抗氧化傷害，細胞毒性，抗發炎，及纖維分解。梔子最主要的活性成份為梔子?，在腸道經細菌代謝成梔子素，此成份可以顯著提升細胞抵抗氧化傷害能力而具抗發炎的功能。由於梔子?與其代謝產物均具抗發炎與抗氧化之效能，因此極有可能對抑制幽門螺旋桿菌感染有其效益。本計畫將在第一年以幽門螺旋桿菌感染之細胞模式測試梔子?代謝產物對抗胃上皮細胞感染幽門螺旋桿菌之能力與其分子機轉，以釐清各代謝產物對治療幽門螺旋桿菌的能力與路徑；並同時篩選可以將梔子 ?水解的細菌，以便用於體外的生物轉換。本計畫將在第二年以幽門螺旋桿菌感染之動物模式探討梔子?之代謝產物對治療感染的能力，並將測試篩選所得細菌的生物轉換效能，以評估細菌之添加能否加強梔子?抑制幽門螺旋桿菌感染之療效，且由監測血液中各代謝產物之含量了解在動物體內轉換之效能。本計畫將在第三年利用具生物轉換效能之細菌大量生產梔子?代謝產物，以提供具療效之成分為臨床用藥之用，此項研究將亦有助於梔子於醫藥上的應用，提高梔子相關產品之市場多樣性及競爭力。近年來，本研究室一直致力於天然食材抑菌能力的探討，也已經發表了相關的研究成果，因此可勝任此一研究。 Gardenia has been used as an herbal medicine to treat liver and gall bladder disorders such as hepatitis and acute jaundice, as well as inflammation and fever in Chinese medicine for many years. The effective biological actions, such as protective activity against oxidative damage, as well as cytotoxic, anti-inflammatory, and fibrolytic activities, have been demonstrated. Since geniposide, the major active compound of gardenia, is transformed into genipin by bacterial enzymes in the intestine and then absorbed, the active components of gardenia, geniposide and genipin also play an important role in antioxiation and antiinflammation. In this proposal, we would like to investigate the effects and machanisms of geniposide and its metoblies in inhibiting Helicobacter pylori infection. This proposal will be finished within 3 years. In the first year, the effects and molecular mechanism of metabolites of geniposide on anti-H. pylori infection will be evaluated in the human gastric cancer epithelial cell culture. And the microorganisms carrying hydrolysis abilities of esters and/or glycosides will be screened for biotransformation study. In the second year, the efficacy of geniposide and its metabolites by oral administration will be studied in the H. pylori infected mice model. The metabolisms and pharacokinetics of geniposide and its metabolites will be investigated. Furthermore, the biotransformation ability of the microorganism that we will screen will be tested in vivo as well. In the third year, after identifying the most effective metabolite of geniposide, the microorgamism –biotransformation system will be optimized by adjusting culture conditions in vitro. Further standardization of the massive production of active compound will be helpful in supplying clinical medication. This research will be improtant to increase the variety of gardenia in medicine application and also enhance the competitiveness of gardenia and its related products. We have been focusing on styding the effects of anti-bacteria activities by natural foods. We have also published several related research data on these studies. Therefore, we are confident to achieve the goal of this study.
顯示於類別:	[生物科技學系暨碩士班] 研究計畫

文件中的檔案:

檔案	描述	大小	格式	瀏覽次數
index.html		0Kb	HTML	129	檢視/開啟

在CMUR中所有的資料項目都受到原著作權保護.

TAIR相關文章

資料載入中.....