| 摘要: | The aim of this study intended to investigate the analgesic, anti-inflammatory and hepatoprotective activities of ethanol extract from Mahonia oiwakensis Hayata (MO) root (MOREtOH) by using acetic acid-induced writhing response, formalin test, λ-carrageenan-induced paw edema and CCl4-induced liver injury model. I also detected the protoberberine alkaloids (berberine, palmatine and jatrorrhizine) contents of MOREtOH by using the high performance liquid chromatography (HPLC) system. While investigating the anti-inflammatory mechanism of MOREtOH, I observed the levels of tumor necrosis factor-α (TNF-α), nitric oxide (NO) in the serum and the neutrophil infiltration, cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) expressions in the edema paw. In order to investigate the hepatoprotective mechanism of MOREtOH, the anti-oxidative enzyme activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GRx), the levels of malondialdehyde (MDA)and neutrophil infiltration in the liver. The levels of TNF-α, NO in the serum were detected as well.
The result showed that MOREtOH (100 and 500 mg/kg) significantly inhibited the acetic acid-induced writhing response and formalin-induced licking time during the later phase. MOREtOH (100 and 500 mg/kg) also significantly decreased the paw edema at the 3th, 4th and 5th h after λ-carrageenan injection. Furthermore, MOREtOH (100 and 500 mg/kg) treatment also significantly increased the levels of TNF-α, NO in the serum and the neutrophil infiltration, COX-2 and iNOS expressions in the edema paw. When rats were treated with CCl4 in the absence of MOREtOH, the activities of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), and the levels of malondialdehyde (MDA) and NO were increased, while the anti-oxidant enzymes activities were decreased. However, when the rats were treated with CCl4 in the presence of MOREtOH, the activities of ALT and AST, and the levels of MDA and NO were significantly reduced, and SOD, GRd and GPx activities were remarkably increased. The contents of berberine, palmatine and jatrorrhizine were 191.45 mg/g extract, 100.15 mg/g extract and 66.45 mg/g extract, respectively.
Taken together, the present result indicated that MOREtOH produced analgesic, anti-inflammatory and hepatoprotective activities. The anti-inflammatory mechanisms of MOREtOH may be related to decreasing the level of COX-2, iNOS and NO production in the edema paw. The hepatoprotective mechanisms of MOREtOH may be related to the inhibition of TNF-α, MDA and NO production via increasing the activities of anti-oxidative enzymes (SOD, GPx and GR). MOREtOH contained protoberberine alkaloids (berberine, palmatine and jatrorrhizine) and may be used as a pharmacological agent in the prevention or treatment of inflammatory and liver disorders. |
