Gallic acid誘發人類胃腺癌細胞凋亡作用及機制之探討

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Title:	Gallic acid誘發人類胃腺癌細胞凋亡作用及機制之探討 A Study on the Apoptosis Induction and Mechanisms in Human gastric epithelial Cells Induced by Gallic acid
Authors:	廖欣怡;Liao, Sin-Yi
Contributors:	醫學檢驗生物技術學系碩士班
Keywords:	細胞凋亡;沒食子酸;Apoptosis;Gallic acid
Date:	2012-08-27
Issue Date:	2012-09-25 14:13:24 (UTC+8)
Publisher:	中國醫藥大學
Abstract:	胃癌是全世界排名第四最常被診斷出來的癌症，而且是所有癌症中死亡率排名第二高。以全世界而言，日本的死亡率排行第一，其次是中南美洲及東歐國家。台灣是胃癌高罹患率地區之一，也是國人好發的癌症之一。好發年齡以40~80歲占大多數，男性又比女性多，然而近年發現年紀在40歲以下亦不在少數。胃癌的成因很多，包括飲食、遺傳、免疫、幽門螺旋桿菌以及其他不明等五種因子共同造成胃癌。沒食子酸，學名為三羥基苯甲酸，為一種酚酸類型的有機酸，又名3，4，5-三羥基酸，化學式為C6H2(OH)3COOH。另外, 沒食子酸是傳統中藥五倍子的主要分許多研究已指出，沒食子酸具有抗癌的功效，可毒殺多種癌細胞。目前治療胃癌的方法通常以手術和化療為主，但我們都知道化療會讓治療者產生嚴重的副作用。本研究目的是在探討五倍子如何抑制AGS胃癌細胞的生長及五倍子的藥理活性深入研究。我們的研究發現，沒食子酸在有AGS胃癌細胞下被活化且能有效的明顯抑制AGS的生長，MTT的結果也顯示有明顯的細胞毒殺效果，接著我們利用cell death ELISA assay 證明沒食子酸能抑制癌細胞的生長。此外，也利用Western blot來觀察細胞凋亡時各個蛋白之間的表現情形,結果發現,沒食子酸會透過Fas來活化 caspase-8來誘發胃癌細胞的細胞凋亡的開始，進而增加促凋亡蛋白Bad, Bak的表現, 使粒線體釋放細胞色素c,接著活化其下游caspase-3及 caspase-9和PARP,使其表現量增加,反之,抗凋亡蛋白Bcl-2的表現量同時也明顯下降, 這些結果都顯示出，沒食子酸在未來具有輔助治療胃癌的潛力。 Gastric cancer is the fourth common to diagnose cancer in the world and is the second highest death rate of all cancer. For worldwide, the death rate of Japan is the highest, second are Central and South America and Eastern European Countries. Gastric cancer is one of the regions of high attack rate in Taiwan and one of the cancer that people easier to occur. The majority of age to 40 to 80 years old are easily to get and male is more than female, but in recent studies showed that also found a few age unnder 40 years old. There are lots of factors to cause gastric cancer, including diet, genetic, immune, Helicobacter pylori, and other unknown factors. Gallic acid is a trihydroxybenzoic acid, a type of phenolic acid, a type of organic acid, also known as 3,4,5-trihydroxybenzoic acid, found in gallnuts, sumac, witch hazel, tea leaves, oak bark, and other plants. The chemical formula is C6H2(OH)3COOH. Many studies have found that gallic acid has anti-cancer effect and cytotoxic to a variety of cancer cells. Most of the current treatment of gastric cancer by surgery and chemotherapy as the main, but we know a great side effects of chemotherapy, the purpose of this study is to determine the Galla Chinensis in AGS gastric cancer cell growth inhibition on the case. And in-depth study of the pharmacological activity of Galla Chinensis, however, we found that the gallic acid activation in AGS gastric cancer cells have a storng inhibition of the growth conditions and cell toxicity by MTT assay. And then, we used the cell death ELISA assay to prove the inhibition of the gallic acid on cancer cell growth. In addition, gallic acid can also lead to cell cycle arrest. Moreover, gallic acid also induced cancer cell apoptosis via activated caspase-3and caspase-8 and caspase-9. These results suggest that gallic acid has the potential for the treatment of gastric cancer.
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