| 摘要: | 由於近年來蓬勃發展的奈米技術，奈米材料是現今正在使用或有可能廣泛應用範圍的物質，其中包括紡織品，裝飾，電子產品，以及在醫療影像，疾病診斷的用途和藥物輸送。
然而，在工業上，具有實際或潛在使用，且未經檢驗的奈米粒子及其數量龐大且不斷增加，必須評估其潛在的毒性及預測方法。由於奈米粒子對免疫系統的毒性作用目前仍不清楚，在這項研究中，使用奈米銀和奈米級氧化鋅，探索對小鼠骨骨髓來源的樹突狀細胞（BM- DCS）的影響。
本研究，利用trypan blue exclusion assay 來探測細胞的毒性、流式細胞儀來探討細胞表面分子的表現量。之後，前處理奈米銀與奈米鋅，濃度分別為：0.1, 1, 10μg/ml或者在同時給予LPS，在24小時之後，利用trypan blue assay，然而，我們並沒有觀察到任何毒性在BM-DCs上，接著，我們調查這兩種奈米粒子對於BM-DCs的成熟是否有影響。結果顯示，我們發現給予在高劑量之下的奈米銀及奈米鋅，會抑制由LPS所誘導活化的BM-DCs，且抑制活化後所表現的表面因子MHC II的表現量。在此研究中，我們發現在給予高劑量下的奈米銀與奈米鋅，抑制了BM-DCs 表面因子的表現。
Due to the booming nanotechnology in recent years, nanomaterials are either being used or have the potential to be widely incorporated in a range of applications, including textiles, finishes, and electronics, as well as having a spectrum of uses in medical imaging, disease diagnoses, and drug delivery. Because of the large and growing numbers of untested nanoparticles and their variants with actual or potential use in industry, there is a need for predictive approaches to assess their potential toxicity. As the toxicity effects of nanoparticles on immune system is still unclear, in this study, the use of nano-silver and nano-zinc oxide to explore the effects on murine bone marrow-derived dendritic cells (BM-DCs) .
Detection of cell viability by trypan blue exclusion assay , cells surface marker by flow cytometry .
After treated with nano-silver and nano-zinc oxide 0.1, 1, 10μg/ml or combination with LPS for 24h, it did not show any obviously cytoxicity effect on BM-DCs by trypan blue assay. Next, we investigated the effects of nanoparticles on BM-DCs maturation. We found that high does nano-silver and nano-zinc oxide both inhibited LPS- induced BM-DCs maturation by decrease in CD80、CD86 and MHC II expressions .
In this study, we found that nano-Ag and nano-ZnO showed decrease in LPS-induced BM-DCs surface marker expression. |
