於囓齒類動物模型探討電針保護古柯鹼引發癲癇之作用：行為及組織免疫化學之研究

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题名:	於囓齒類動物模型探討電針保護古柯鹼引發癲癇之作用：行為及組織免疫化學之研究 Electroacupuncture protects cocaine-induced seizure in a murine model: a behavior and immunohistochemistry study
作者:	尹柏力;Ivanic, Boris
贡献者:	國際針灸碩士學位學程
关键词:	電針;古柯鹼;c-fos;古柯鹼引起之相關症狀;electroacupuncture;cocaine;c-fos;cocaine-induced seizure
日期:	2012-07-31
上传时间:	2012-08-31 16:32:55 (UTC+8)
出版者:	中國醫藥大學
摘要:	本研究以免疫組織化學方法來探討電針(electroacupuncture, EA)對古柯鹼(cocaine，75 mg/kg)在小鼠腦中造成c-fos表現之影響。在固定動物姿勢與不施以麻醉的狀況下，將實驗分為三組：(1)控制組：不施行電針；(2)電針組：於動物GV14(大椎)與GV20(百會)施行電針(50 Hz ; 1mA)和(3)假電針組：電針動物不具穴位之尾巴(非穴位)。電針完一分鐘後，於腹腔注射古柯鹼(75 mg/kg)。古柯鹼注射兩小時後，犧牲所有實驗動物並利用免疫組織化學的方法觀察其腦中c-fos的表現狀態。實驗結果發現，在電針GV14與GV20的電針組可降低古柯鹼對於小鼠所引起的毒性症狀，並減低下丘腦外側區和丘腦的腦室壁核中由古柯鹼所引起的c-fos表現。 We tested the effects of electroacupuncture (EA) on seizures induced by single administration of cocaine (75 mg/kg) in mice and followed by an imunohistochemistry study to determine the c-fos expression in the brain. Three groups of animals were included, i.e. (1) control group: animals were strained without EA, (2) EA treatment group: animals were strained and received EA (50 Hz; 1mA) for 15 min at GV14 (Dazhui) and GV20 (Baihui) without anesthesia before cocaine injection and (3) sham acupuncture group, where EA was performed on the tail (non-acupoints) of the animal. EA treatments were followed 1 min afterwards by an intraperitoneal injection of cocaine (75 mg/kg). At two hours after cocaine injection, all groups were sacrificed and the c-fos express in the brain was determined by an immunohistochemistry method. In addition, we also examined the c-fos expression after applicationof EA to GV14 and GV20, without cocaine injection. We found that EA pretreatment at GV14 and GV20 attenuated the severity of cocaine-induced seizure in EA group and decreased cocaine-induced c-fos expression in paraventrical nucleus of thalamus and lateral hypothalamic area.
显示于类别:	[針灸研究所] 博碩士論文

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