乳酸菌對於梔子萃取物治療小鼠感染幽門螺旋桿菌的影響

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題名:	乳酸菌對於梔子萃取物治療小鼠感染幽門螺旋桿菌的影響 Effects of Lactobacillus spp. on improving the efficiency of gardenia extract in treating Helicobacter pylori infection in mice
作者:	賴彥汝;Lai, Yen-Ju
貢獻者:	生物科技學系碩士班
關鍵詞:	幽門螺桿菌;梔子;乳酸菌;Helicobacter pylori;gardenia;Lactobacillus spp.
日期:	2012-08-02
上傳時間:	2012-08-31 16:30:13 (UTC+8)
出版者:	中國醫藥大學
摘要:	在世界上約有 50 % 人口感染幽門螺桿菌，而幽門螺桿菌與慢性胃炎、胃潰瘍和胃癌有關聯。70 % 至95 % 的消化性潰瘍患者都受其感染。梔子在中醫上，被用來治療肝、膽囊疾病以及炎症和發燒。研究上也證實，梔子對抗氧化損傷，以及細胞毒性和抗發炎活性的作用。梔子苷是梔子果實中的主要環烯醚萜苷，可在腸道中經細菌酶轉化成梔子素。本研究發現，梔子素毒殺幽門螺桿菌的能力較梔子苷高20倍以上，並且梔子素可有效降低幽門螺桿菌毒力因子 vacA基因的表現，改變幽門螺桿菌型態，降低侵入胃上皮細胞的能力，對幽門螺桿菌感染引發的發炎反應與氧化壓力亦可有效抑制。因此，微生物酵素對藥物活性具重要影響。我們由 68 株乳酸菌中篩選出擁有最強 β-葡萄糖苷酶活性的分離株 Lactobacillus rhamnosu JB3。利用 JB3 轉換梔子萃取物之轉換液與梔子素具相同生物活性。並在小鼠感染實驗中證實，同時服用 JB3 與梔子萃取物較單純口服梔子萃取物，更能抑制感染胃組織中 vacA 基因之表現量，並降低幽門螺旋桿菌特異抗體 IgA 、 IgG 與 IgM 在血清中的表現量，及抑制血清中因感染引發的 IFN-γ 的表現量。藉此研究，可降低抗藥菌株的產生，並減輕治療副作用的發生，提供了治療幽門螺旋桿菌感染的新興治療方式。 Helicobacter pylori infection is associated with chronic gastritis, peptic ulcers, and gastric cancer. About 50% of the population in the world is infected by H. pylori. Furthermore, 70% to 95% of H. pylori-infected patients are suffering from peptic ulcer. Gardenia has been used as an herbal medicine to treat liver and gall bladder disorders such as hepatitis and acute jaundice, as well as inflammation and fever in Chinese medicine for many years. The effective pharmacological actions, such as protective activity against oxidative damage, as well as cytotoxic, and anti-inflammatory activities, have been demonstrated. Geniposide is one of the major iridoid glycosides in gardenia fruit and is transformed into genipin by bacterial enzymes in the intestine and then absorbed. In our study, it has showed that the anti-H. pylori activity of genipin was as least 20-fold higher than that of geniposide. And genipin could also decress the mRNA expression of vacA gene and the invasion ability of H. pylori, and change the morphoragy of bacteria. Infection-induced inflammation and oxidactive stress were suppressed by it. Therefore, the biotransformation by bacterial enzymes is important for the bioactivity of herbal compounds. We screened 68 filed-isolates, Lactobacillus rhamnosu JB3 showed the best β-glucosidase. Thus, JB3 was used to convert gardenia extract (GE) into genipin. The converted GE showed similar bioactivities to genipin. In mouse-infecion model, the treatment combined JB3 and GE could significantly decrease H. pylori infection and H. pylori-specific IgA, IgG, and IgM levels, and also infection-induced IFN-γexpression in serum. This design could not only provide a new treatment of H. pylori infection and also over come the problem that antibiotic treatment caused by disturbing the proper function of the intestinal flora.
顯示於類別:	[生物科技學系暨碩士班] 博碩士論文

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