中國菟絲子對小鼠鎮痛及抗發炎作用機轉之研究

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Title:	中國菟絲子對小鼠鎮痛及抗發炎作用機轉之研究 Studies on the analgesic and anti-inflammatory mechanisms of Cuscuta chinensis Lam. in mice
Authors:	趙威凱;Chao, Wei-Kai
Contributors:	中國藥學暨中藥資源學系碩士班
Keywords:	鎮痛;抗發炎;中國菟絲子;醋酸扭體試驗;福馬林舔足實驗;λ-角叉菜膠誘發小鼠足蹠浮腫試驗;analgesic;anti-inflammatory;Cuscuta chinensis Lam.;acetic acid-induced writhing response;formalin tests;λ-carrageenan- induced paw edema in mice
Date:	2012-07-27
Issue Date:	2012-08-31 16:11:08 (UTC+8)
Publisher:	中國醫藥大學
Abstract:	中國菟絲子是台灣傳統中醫常用滋補中藥之一，其基原為旋花科植物中國菟絲子 [Cuscuta chinensis (Lam.)]的種子。常被用來治療胎動不安、大小便失禁、高血壓、腎虛等症狀。有關中國菟絲子的研究文獻指出具有抗氧化能力和增強人體免疫力，但對抗發炎與鎮痛能力的探討深入未盡完全。此研究以醋酸扭體試驗以及福馬林舔足實驗做為探討其中國菟絲子之鎮痛作用，並以λ-角叉菜膠誘發小鼠足蹠浮腫試驗來探討其中國菟絲子之抗發炎作用。並檢測其腳組織內MDA、NO、COX-2、NF-κB、TNF-α、IL-1β、IL-6，藉以探討抗發炎分子的機轉；檢測其肝組織內SOD、GPx、GRd抗氧化酵素的活性。上述結果顯示，中國菟絲子甲醇萃取物能有效抑制醋酸扭體次數、福馬林前期中樞性與後期發炎性疼痛作用以及λ-角叉菜膠誘發小鼠足蹠浮腫的反應，並有效減少發炎因子 (MDA、NF-κB、TNF-α、IL-1β、IL-6、NO、COX-2)的含量，並提昇肝臟抗氧化酵素 (SOD、GPx、GRd)的活性。 A member of the Convolvulaceae family, seeds of Cuscuta chinensis (Lam.) called Chinese Dodder are commonly used as a tonic medicinal material and used for treating tocolysis, incontinence, high blood pressure and kidney deficiency by traditional Chinese physicians in Taiwan. In previous studies, the extracts of C. chinensis exhibit efficient antioxidative and human immunity enhancement activities. However, those researches of the anti-inflammatory and analgesic effects of Cuscutae Semen are still not enough thorough. In this study, the anti- inflammatory and analgesic effects of the methanolic extract of C. chinensis (CCMeOH) were examined by acetic acid-induced writhing response and formalin tests. The anti-inflammatory effect of the CCMeOH was examined by λ-carrageenan- induced paw edema in mice, and the indomethacin was used as a positive control among these animal experiments. To understand the mechanism of anti- inflammatory effect of the CCMeOH, the superoxide dismutase (SOD), glutahoine reductase (GSH-Rx), glutahoine peroxidase (GSH-Px) activities in livers and malondialdehyde (MDA), nitric oxide (NO), cyclooxygenase 2 (COX-2), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), tumor necrosis factor-α (TNF-α), interleukin-1-beta (IL-1β), and interleukin-6 (IL-6) levels in paws were evaluated by ELISA kit. The result showed that CCMeOH inhibited the acetic acid-induced writhing response, the early phase and late phase of formalin- induced licking times and Carrageenan-induced rat paw oedema. CCMeOH decreased those inflammatory factors (MDA、NF-κB、TNF-α、IL-1β、IL-6、NO、COX-2) and increased the ability of those antioxidant enzymes (SOD、GPx、GRd).
Appears in Collections:	[Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources] Theses & dissertations

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