The use of antimicrobial agents has resulted in the increase of antimicrobial-resistant bacteria in humans and animals. The presence of fluoroquinolone-resistant Salmonella enterica serovar Choleraesuis (S. Choleraesuis) especially raised the big public health concern, as food animals were argued to be the source of the infection in humans. Therefore, it is of urgent need to develop a quick diagnosis for S. Choleraesuis with quinolone resistance for better disease control. In this project, we plan to collect S. Choleraesuis isolates for molecular identification and to characterize their genetic patterns. The results could be helpful to identify mutation sites associated with quinolone resistance and be further applied to explore the relationship of S. Choleraesuis in humans and animals. The final goal of this project is to use the identified mutation sites to develop quick molecular diagnosis to differentiate quinolone-resistant S. Choleraesuis from the susceptible ones.
