探討植物肉桂中生物活性成分α-水芹烯誘導小鼠血癌和人類血癌細胞株(WEHI-3與U937)產生細胞凋亡與正常和血癌小鼠中免疫調控之離體與活體之研究

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題名:	探討植物肉桂中生物活性成分α-水芹烯誘導小鼠血癌和人類血癌細胞株(WEHI-3與U937)產生細胞凋亡與正常和血癌小鼠中免疫調控之離體與活體之研究
作者:	鍾景光(Jing-Gung Chung);周淑姿;楊美都(Mei-Due Yang)
貢獻者:	生命科學院生物科技學系;中國附醫醫學研究部
關鍵詞:	α-phellandrene;murine and human leukemia cells;apoptosis;DNA microarray;leukemia mice;immunodeficiency mice;aging mice;immunemodulatioins;in vitro;in vivo
日期:	2012-07-31
上傳時間:	2012-06-13 10:53:28 (UTC+8)
摘要:	探討植物肉桂中生物活性成分α-水芹烯誘導小鼠血癌和人?血癌細胞株(WEHI-3 與 U937)產生細胞凋亡與正常和血癌小鼠中免疫調控之?體與活體之研究 α-水芹烯(α-phellandrene)，為辛香?及香草植物中常?之活性成份，如：中藥中的肉桂、生薑、 ?薄荷、艾?、大蒜、細辛、松?……等，且許多文獻指出其有許多生物活性的效果。但目前α-水芹烯對血癌則無相關之?體與活體之抗癌活性研究。因此，本計畫之研究目的主要在探討α-水芹烯對小鼠血癌細胞株WEHI-3 及人?血癌細胞株U937 進??體(in vitro)及活體(in vivo)抗血癌評估及免疫調節的研究。擬分為三主題並預期三?內完成。第一?計劃目的是：(1) 探討α-水芹烯對小鼠血癌細胞株(WEHI-3)之細胞週期停滯及細胞凋亡的影響。(2) 探討α-水芹烯對WEHI-3 細胞對於細胞週期停滯和細胞凋亡相關蛋白質與基因層次的影響。(3) 建?BALB/c 小鼠血癌模式，檢測α-水芹烯對於抗 WEHI-3 小鼠血癌之抗血癌評估。(4) 檢測α-水芹烯對於抗血癌小鼠之免疫調節之評估。第二?計劃目的是：(1) 檢測α-水芹烯對人?血癌細胞(U937)之細胞週期停滯及細胞凋亡相關?徑的影響。(2) 檢測α-水芹烯對U937 細胞對於細胞凋亡相關蛋白質與基因層次(內在?徑、外在?徑與內質網壓?)的影響。(3) 檢測α-水芹烯對於人?血癌U937 ?種移植於BALB/c ?小鼠腫瘤之影響評估。(4) 檢測α- 水芹烯對於?種移植腫瘤之凋亡相關蛋白質與基因層次評估。第三?計劃目的是：?用正常小鼠、? 小鼠(T 細胞缺陷)與?化小鼠進?α-水芹烯對小鼠免疫調節之影響，評估項目如：(1) 小鼠外觀與重? 評估。(2) 小鼠週邊單核球細胞評估(細胞族群評估、血清中細胞激素與吞噬細胞吞噬活性)。(3) 小鼠脾臟細胞評估(T 與B 細胞增殖、自然?手細胞毒?活性和細胞激素檢測)。(4) 小鼠血清中，生化檢測評估安全性。此??藉由這些檢測進??解α-水芹烯在提升正常小鼠、免疫功能缺失小鼠與?齡化小鼠免疫功能之探討。 Investigations of α-phellandrene, a bioactive compound from Cinnamon, in induction of apoptotic cell death in murine and humam leukemia cell lines (WEHI-3 and U937) and immune modulation in normal and leukemia mice in vitro and in vivo. Alpha-phellandrene is a common and active compound from spicy and aromatic plants including traditional Chinese medicine cinnamon, ginger, green mint leaves, garlic, Asarum, pine needles and so on. It is reported that α-phellandrene exhibited many biological activities; however, there is no available information addressing α-phellandrene-affected biological and anticancer activity on leukemia in vitro and in vivo. Therefore, the purpose of this project was to investigate the α-phellandrene on the murine leukemia cell line (WEHI-3) and human leukemia cell line (U937) in vitro, anti-leukemia activity and immunemodulations in vivo. This proposal can be divided into 3 subjects and should be finished within three years. Subject I in the first year is to investigate 1) the effects of α-phellandrene-induced cell cycle arrest and apoptosis in murine leukemia WEHI-3 cells in vitro; 2) the effects in cell cycle arrest and apoptosis-associated protein and gene expression levels in WEHI-3 cells; 3) estabolishment of leukemia mice and investigations of α-phellandrene-affected leukemia in vivo; 4) influences of immune response in leukemia mice in vivo. Subject II in the second year is to investigate 1) the effects of signaling pathways in α-phellandrene-induced cell cycle arrest and apoptosis in human leukemia U937 cells in vitro; 2) the effects in apoptosis-associated protein and gene expression levels (extrinsic pathway, intrinsic pathway and ER stress) in U937 cells; 3) investigations of α-phellandrene-affected murine xenograft leukemia transplantation tumor in vivo; 4) influences of apoptosis-associated protein and gene expression levels from transplantation tumor after α-phellandrene exposure in vivo. Subject III in the third year is to investigate the effects of immune responses in α-phellandrene-treated normal mice, nude mice (T-cell deficiency) and aging mice in vivo. Assessment projects such as: 1) appearance and weight of mice; 2) assessment of peripheral mononuclear blood cells including cell population, cytokines serum and phagocytic activity; 3) determinations of splnocytes from mice, such as T and B-cell proliferations, natural killer cell cytotoxic activity and cytokine; 4) biochemical examinations in serum is to assess safety. Based on these determinations in this year, it is known that α-phellandrene promoted the immune responses in the normal mice, immunodeficiency mice and aging mice in vivo.
顯示於類別:	[生物科技學系暨碩士班] 研究計畫

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