Effects of chitosan oligosaccharides on drug-metabolizing enzymes in rat liver and kidneys.

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Title:	Effects of chitosan oligosaccharides on drug-metabolizing enzymes in rat liver and kidneys.
Authors:	姚賢宗(Hsien-Tsung Yao);(Mei-Ning Luo);(Lang-Bang Hung);(Meng-Tsan Chiang);(Jia-Hsuan Lin);(Chong-Kuei Lii);黃俊瑩(Huang, Chun-Yin)
Contributors:	健康照護學院營養學系
Keywords:	Chitosan oligosaccharides;Drug-metabolizing enzymes;Oxidative;Liver;Kidneys;Rats
Date:	2012-02
Issue Date:	2012-04-18 12:42:00 (UTC+8)
Abstract:	To investigate the effect of chitosanoligosaccharides (COS) on drug-metabolizingenzymes in ratliver and kidneys, male Spraque–Dawley rats were fed a diet containing 1% or 3% COS for 5 weeks. The activities of cytochrome P450 (CYP) enzymes, UDP-glucurosyltransferase (UGT) and glutathione S-transferase (GST) in the liver and kidneys were determined. Significant decreases in microsomal CYP3A-catalyzed testosterone 6β-hydroxylation, CYP2C-catalyzed diclofenac 4-hydroxylation, and CYP4A-catalyzed lauric acid 12-hydroxylation in the liver of rats fed the COS diets were observed compared with those rats fed the control diet. Immunoblot analyses of CYP proteins showed the same trend as with enzyme activities. Increased glutathione content in liver was found in rats fed the 1% COS diet. Increased hepatic NADPH: quinone oxidoreductase 1 (NQO1) activity was found in rats fed the COS diets. In kidneys, COS had little or no effect on CYP enzyme activities. However, increased GST activity was observed in rats fed the COS diets. Moreover, a higher UGT activity was found in rats fed the 1% COS diet. Our results indicate that COS may suppress hepatic CYP enzymes and induce phase II detoxifying reactions in the liver and kidneys of rats.
Relation:	FOOD AND CHEMICAL TOXICOLOGY 50(5):1171-1177
Appears in Collections:	[Graduate Institute of Nutrition ] Journal articles

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