Simultaneous determination of components released from dental composite resins in human saliva by liquid chromatography/multiple-stage ion trap mass spectrometry

CMUR > College of Chinese Medicine > School of Chinese Medicine > Journal articles > Item 310903500/43801

Please use this identifier to cite or link to this item: http://ir.cmu.edu.tw/ir/handle/310903500/43801

Title:	Simultaneous determination of components released from dental composite resins in human saliva by liquid chromatography/multiple-stage ion trap mass spectrometry
Authors:	許維儀(Wei-Yi Hsu);王文忻(Ven-Shing Wang);賴建成(Chien-Chen Lai);蔡輔仁(Fuu-Jen Tsai)
Contributors:	中醫學院中醫學系學士班中醫婦兒學科;中國附醫基因醫學部
Keywords:	Biodegradation;Dental composite resin;LC-MS/MS
Date:	2012-03
Issue Date:	2012-04-18 12:20:50 (UTC+8)
Abstract:	Dental composite resins are widely used for fixing teeth; however, the monomers used in dental composite resins have been found to be cytotoxic and genotoxic, namely triethylene glycol dimethacrylate (TEGDMA), urethane dimethacrylate (UDMA), and bisphenol A glycol dimethacrylate (Bis-GMA). In this study, we incubated dental composite resins with human saliva for demonstrating the released monomers and biodegradation products. A simple saliva sample dilution method without purification or derivatization was used for quantification. We found that liquid chromatography coupled with multiple-stage ion trap mass spectrometry (LC-MSn) operated in selected reaction monitoring (SRM) mode was able to separate the three monomers within 10 min. The calibration curves were linear (R2>0.996) over a wide range for each monomer in saliva: TEGDMA, 5–500 ppb; UDMA, 5–100 ppb, and Bis-GMA, 5–700 ppb. Furthermore, several biodegradation products were discovered with data-dependent MS/MS scan techniques. Although TEGMA degradation products have previously been reported, we identified two previously unknown UDMA degradation products. The LC-MS/MS method developed in this study was able to successfully quantify monomers and their principal biodegradation products from dental composite resins in human saliva.
Relation:	ELECTROPHORESIS 33(4):719-725
Appears in Collections:	[School of Chinese Medicine] Journal articles

Files in This Item:

File	Description	Size	Format
index.html		0Kb	HTML	249	View/Open

Loading...