IL-10-1082 Nucleotide Polymorphism and Risk of Non-small Cell Lung Cancer

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Title:	IL-10-1082 Nucleotide Polymorphism and Risk of Non-small Cell Lung Cancer IL-10-1082核甘酸多型性與非小細胞肺癌的風險性
Authors:	李仁智(Jen-Chih Lee);施純明(Chuen-Ming Shih)*;李瑤玲(Yao-Ling Lee);邱慧玲(Hui-Ling Chiou)
Contributors:	北港附設醫院內科部胸腔內科
Keywords:	Interleukin-l;基因多型性;非小細胞肺癌;interleukin-10;polymorphism;non-small cell lung cancer
Date:	2005-12
Issue Date:	2009-08-20 20:08:34 (UTC+8)
Abstract:	前言：在正常細胞或是腫瘤細胞可釋放出IL-10 (Interleukin-10)。IL-10是一種主要抗發炎的細胞激素，它和自體免疫疾病，移植耐受性與腫瘤發生等有相關性。個別間的IL-10變化和IL-10啟動子(promoter)區域的基因多型性有關。本研究目的是來探討IL-10啟動子的基因多型性與非小細胞肺癌發生的關聯性。方法：我們收集了一百二十位非小細胞肺癌病人與135位年紀、性別相符合的健康吸煙者來進行比較。在由周邊淋巴細胞上分離出的基因體序列用聚合脢鍊反應(PCR; polymerase chain reaction)－片段長度多型性(fragment length polymorphism technique)技術來分析IL-10啟動子區的基因多型性。我們用聚合脢鍊反應產品的直接定序(direct sequencing)來證實這項技術的有效性。接下來用統計分析來探索IL-10啟動子的基因多型性在非小細胞肺癌病人的敏感性分佈。結果：IL-10-1082的基因型分佈頻率在非小細胞肺癌病人與控制組上有明顯的不同。在非小細胞肺癌病人身上的IL-10-1082 GG/GA基因型的出現頻率明顯高於AA基因型[OR=5.82, 95% CI 2.88-12.0, p<0.0001]。結論：IL-10的基因多型性和非小細胞肺癌kg的發生有顯著的相關性。 Study objectives: Interleukin-10 (IL-10) is mainly an anti-inflammatory cytokine that is produced by a number of cells, including normal and neoplastic cells, and has been implicated in autoimmunity, transplantation tolerance, and tumorigenesis. Inter-individual variations in IL-10 production genetically contributed to polymorphisms within the IL-10 promoter region. The aim of this study was to determine whether polymorphisms in the IL-10 gene promoter were involved in predisposing an individual to non-small cell lung cancer (NSCLC). Patients: A total of 120 patients with non-small cell lung cancer were recruited into this study, together with 135 age-and gender-matched healthy smokers acting as control subjects. Measurements: Polymorphisms of sites within the promoter region of the IL-10 gene were analyzed using a polymerase chain reaction-restriction fragment length polymorphism technique on genomic DNA isolated from peripheral lymphocytes. The validity of this technique was proven by direct sequencing of polymerase chain reaction products. Statistical analyses were conducted to explore the contribution of the polymorphism of the IL-10 promoter to the susceptibility to NSCLC. Results: The distribution frequencies of the genotype of IL-10-1082 were significantly different between NSCLC patients and controls. Higher ORs for NSCLC were seen for individuals with IL-10-1082 GG/GA genotypes against the AA genotype [OR=5.82, 95% Cl 2.88-12.0, p<0.0001]. Conclusion: The polymorphism of the IL-10 gene was significantly associated with the occurrence of NSCLC.
Relation:	胸腔醫學20(6):591~598
Appears in Collections:	[China Medical University Beikang Hospital] Journal articles

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