紅花乙醇萃取物對大鼠後肢肌肉缺血-再灌流損傷模式之保護作用

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题名:	紅花乙醇萃取物對大鼠後肢肌肉缺血-再灌流損傷模式之保護作用 Protective effect of ethanol extract from Carthamus tinctorius L. on ischemia/reperfusion injury in rat hind limb model
作者:	施靜妤
贡献者:	藥學系碩士班
关键词:	�紅花乙醇萃取物(ECT);Hematoxylin and Eosi(H&E)染色;超氧化物歧化酶(SOD);IL-1β ischemia/reperfusion;Carthamus tinctorius L. (ECT);Hematoxylin and Eosin (H&E) stain;superoxide dismutase (SOD);IL-1β
日期:	2011-07-15
上传时间:	2011-10-17 17:03:46 (UTC+8)
出版者:	中國醫藥大學
摘要:	缺血-再灌流傷害是一個複雜的病理學，伴隨著自由基與嗜中性白血球的產生。紅花乙醇萃取物是由紅花（Carthamus tinctorius L.）萃取而得的，含有抗氧化、抗發炎及清除自由基之功能，也包括了各種藥理作用。本研究旨在研究紅花乙醇萃取物對於大鼠後肢肌肉缺血-再灌流傷害之保護效果。將大鼠隨機分組，實驗未進行肌肉缺血前給予不同劑量的紅花乙醇萃取物或Normal saline（N/S），造成缺血2小時，鬆開止血帶形成再灌流傷害。在缺血前與再灌流後分別測量血漿NO、IL-1β、TNF-α濃度；取左右腿的內縮肌切片做H&E染色；測定SOD、GPx、GR、MDA和MPO活性。結果顯示，事前給予紅花乙醇萃取物依劑量低至高，對於缺血-再灌流誘導大鼠後肢內縮肌的傷害可能藉由紅花乙醇萃取物之抗氧化和抗發炎的能力，具有降低內縮肌組織傷害之效果。 Ischemic/reperfusion (I/R) injury is a complex pathophysiology with evident increase in free radical production and neutrophil infiltration. The ethanol extracts from Carthamus tinctorius L., extracted from the Carthamus tinctorius L., has various pharmacological effects, including anti-inflammation, anti-oxidation and free radical scavenger effects. In this study, we examined the effect of the ethanol extracts from Carthamus tinctorius L. on ischemia/reperfusion (I/R) injury in rat hind limb muscle. Two hours of ischemia were introduced to right hind limb by applying inelastic rubber tourniquet to the proximal thigh. Muscle damage was evaluated in 4 groups, including control and pretreatment groups. The ethanol extracts from Carthamus tinctorius L. was injected by IP. before tourniquet properly tied limbs of rat for 30 min, after 2-hours ischemia, losen the tourniquet to reperfusion 22-hours. Plasma NO, IL-1β, TNF-α concentration before ischemia and after reperfusion was measured. Hematoxylin and Eosin (H&E) stain, superoxide dismutase (SOD), glutathione Peroxidase (GPx), glutathione Reductase (GR), malondialdehyde (MDA), and myeloperoxidase (MPO) was also evaluated in skeletal muscles. Our results suggest treatment of the ethanol extracts from Carthamus tinctorius L. shows great potential for anti-inflammatory, and anti-oxidation in ischemia/reperfusion injury musculus adductormagnus.
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