CTGF藉由影響COX-2的表達而抑制口腔癌細胞移行能力

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 醫學院 > 醫學檢驗生物技術學系暨碩士班 > 博碩士論文 > Item 310903500/41419

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.cmu.edu.tw/ir/handle/310903500/41419

题名:	CTGF藉由影響COX-2的表達而抑制口腔癌細胞移行能力 CTGF inhibits cell motility and COX-2 expression in oral cancer cells
作者:	楊婉瑜
贡献者:	醫學檢驗生物技術學系碩士班
关键词:	口腔癌;環氧化酵素;結締組織生長因子 oral cancer;cox-2;CTGF
日期:	2011-07-25
上传时间:	2011-10-17 16:56:57 (UTC+8)
出版者:	中國醫藥大學
摘要:	口腔鱗狀細胞癌(SCC)是屬於高度侵襲惡性轉移的癌症。COX-2環氧化酵素是屬於誘導型的前列腺素合成酶，和癌症的轉移有相當大的關係。結締組織生長因子(CTGF)，是屬於一種分泌型的細胞激素會和Integrin結合進而調控人類癌症細胞的侵襲。但CTGF對人類口腔癌細胞轉移能力的影響則尚未見到文獻報導。我們發現CTGF會減低口腔癌細胞migration的能力和降低COX-2的表現。?庮?????nintegrin單株抗體，PI3K抑制劑(Ly294002 and wortmannin)和AKT的抑制劑(AKT inhibitor)會反轉CTGF抑制口腔癌細胞migration的能力和COX-2的表現。在CTGF刺激下會減少FAK，PI3K，AKT磷酸化。除此之外，c-Jun siRNA也具有拮抗CTGF抑制細胞轉移能力和COX-2的表現。CTGF減少c-Jun結合到COX-2 Promoter上之AP-1 element。綜合以上結果，CTGF經由?庮??5 integrin receptor抑制 FAK，PI3K，AKT的訊息傳導，進而抑制c-Jun活性且減少COX-2表現，而抑制人類口腔鱗狀細胞癌的轉移。 Oral squamous cell carcinoma (SCC) has a striking tendency to migrate and metastasize. Cyclooxygenase (COX)-2, the inducible isoform of prostaglandin synthase, has been implicated in tumor metastasis. Connective tissue growth factor (CTGF), a secreted protein that binds to integrins, modulates the invasive behavior of certain human cancer cells. However, the effect of CTGF on migration activity and COX-2 expression in human oral cells is mostly unknown. Here we found that CTGF reduced the migration and expression of COX-2 in human oral cancer cells.?n ?庮??5 integrin?叮onoclonal antibody, phosphatidylinositol 3-kinase inhibitor (Ly294002 and wortmannin) and Akt inhibitor reversed the migration activity and COX-2 expression which were inhibited by CTGF. CTGF stimulation decreased the phosphorylation of focal adhesion kinase (FAK), PI3K and Akt. In addition, c-Jun siRNA also antagonized the CTGF-inhibited migration and COX-2 expression. Moreover, CTGF decreased the binding of c-Jun to the AP-1 element on the COX-2 promoter. Taken together, our results indicated that CTGF inhibits the migration of oral cancer cells by decreasing COX-2 expression through the ?庮??5 integrin receptor, FAK, PI3K, Akt, c-Jun and AP-1 signal transduction pathway.
显示于类别:	[醫學檢驗生物技術學系暨碩士班 ] 博碩士論文

文件中的档案:

档案	描述	大小	格式	浏览次数
index.html		0Kb	HTML	44	检视/开启

在CMUR中所有的数据项都受到原著作权保护.

TAIR相关文章

数据加载中.....