沈香醇的人體角質層滲透在不同暴露條件下之比較

CMUR > College of Public Health > Department of Public Health > Thesis & dissertation > Item 310903500/41286

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Title:	沈香醇的人體角質層滲透在不同暴露條件下之比較 The skin permeability of linalool to human stratum corneum in different exposure pattern
Authors:	林子淵
Contributors:	公共衛生學系碩士班
Keywords:	沈香醇;貼布法;體內試驗;皮膚暴露;分配係數;擴散係數;角質層厚度;皮膚障壁功能 linalool;tape stripping;in vivo;skin exposure;partition coefficient;diffusion coefficient;stratum corneum thickness;skin barrier function
Date:	2011-07-27
Issue Date:	2011-10-17 16:16:10 (UTC+8)
Publisher:	中國醫藥大學
Abstract:	沈香醇（linalool）為一種常用的芳香物質，也是藥物皮膚暴露研究常探討的輔助劑之一且在許多種精油成分中都有沈香醇的存在。尤其在芳香產品上常添加沈香醇，而這些產品多是與皮膚有直接的接觸，因此在日常生活周遭有很大的機會接觸到沈香醇。皮膚是人體的第一道防線，主要功能稱為皮膚障壁功能，而其與皮膚健康、化學物質吸收及皮膚疾病有關。過去有文獻證實沈香醇接觸過量會造成皮膚發炎，且會對皮膚障壁功能造成影響。本研究以自願受試者方式進行皮膚滲透探討，其目的主要為：（1）不同暴露濃度、時間條件下linalool在不同角質層深度之濃度分布情況。（2）推估Linalool的分配係數（partition coefficient）、擴散係數（Diffusion coefficient）。（3）Linalool對皮膚障壁完整度之影響。本研究以問卷方式篩選合格受試者，共18名自願受試者，在三種暴露時間10分鐘、20分鐘、30分鐘及三種暴露濃度10%、15%、20%之條件下利用貼布法進行皮膚採樣並分析角質層厚度及該貼布中沈香醇含量。皮膚障壁功能測試方面，以4位自願受試者，進行皮膚暴露實驗，使用貼布法採樣並測量經皮水分散失，以貼布次數做比較未暴露組及暴露組之差別。結果顯示受試者其常態化角質層介於10.0 %至47.7 %。在分析方面，結果顯示沈香醇在角質層中含量會隨濃度上升而增加，其分配係數K與擴散係數D則沒有因時間、濃度變化而改變。在皮膚障壁功能實驗方面，結果顯示沈香醇會造成經皮水分散失上升。綜合結果與討論，結論為沈香醇會因暴露濃度增加，其滲透量也會增加。在不同深度角質層下，沈香醇之K、D值會隨角質層深度增加而減少。沈香醇確實造成經皮水分散失增加進而損害皮膚障壁功能。 Linalool is a commonly used aromatic substance, and it is one of the percutaneous penetration enhancers study in skin exposure, and is one of the components in many essential oils. Especially in the aromatic products often add linalool, and these products are mostly direct contact with the skin. Therefore, people have a high possibility of contact with linalool in environment. Skin is the first part of body defense system, which the major function is called skin barrier function, and that affects skin health, chemical absorption, and skin diseases. In the past, some references prove contact with too much linalool will cause skin inflammation and influence skin barrier function. This study will investigate the skin permeability of linalool by volunteers, the objectives for three. First, to investigate the concentration distribution in different stratum corneum depth of linalool exposed different exposure pattern. Second, to use linalool exposed experimental data to estimate the partition coefficient and diffusion coefficient. Finally, to investigate the effect of linalool exposed skin barrier relate to skin integrity. In this study, will use questionnaire to select for the experimental subjects, whole are 18 healthy volunteers. The experimental conditions are three exposed time and three concentrations, whole are 10 minute, 20 minute, 30 minute, and concentration of 10%, 15%, and 20%. In these conditions, the experiment will use tape stripping to sample human skin and measure TEWL. And then, we analyze the skin samples, and estimate the stratum corneum thickness with TEWL value. In skin barrier function estimation experiment selects 4 healthy volunteers for skin exposed experiment, the sampling method also use tape stripping and measure TEWL. The unexposed group and exposure groups use tape numbers to interpret the variation. The stratum corneum estimation results indicate the volunteer’s normalization of the stratum corneum are 10.0% to 47.7%. In chemical and data analysis result shows that linalool mass in stratum corneum will increase by concentration rising. Furthermore, the partition coefficient has no variation when the exposure concentration rises and exposure time increased. In skin barrier function estimation experiment show TEWL will increase by skin exposed to linalool. In conclusions, linalool permeability will increase by concentration rising. In different stratum corneum thickness, the partition coefficient and diffusion coefficient of linalool will decrease by stratum corneum thickness increase. Finally, the skin barrier function will damage by skin exposed to linalool.
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