中國醫藥大學機構典藏 China Medical University Repository, Taiwan:Item 310903500/4123
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    题名: Gypenosides Inhibited N-acetylation of 2-aminofluorene, N-acetyltransferase Gene Expression and DNA Adduct Formation in Human Cervix Epithelioid Carcinoma Cells
    作者: 邱燦宏(Tsan-Hung Chiu);陳榮洲(Jung-Chou Chen);陳立德;李昭宏(Jau-Hong Lee);鍾景光(Jing-Gung Chung)*
    贡献者: 中醫學院中醫學系學士班中醫內科學科;中國附醫婦產部
    日期: 2004-12
    上传时间: 2009-08-20 19:29:10 (UTC+8)
    摘要: N-acetylation plays an important role in the metabolism of arylamine drugs and carcinogens and is catalyzed by cytosolic N-acetyltransferase (NAT). Gypenosides are the major components of Gynostemma pentaphyllum Makino which had been used as a natural folk medicine in the Chinese populations. Gypenosides were selected for examining the inhibition on the N-acetylation of 2-aminofluorene (AF), DNA-AF adduct formation and NAT gene expression in the human cervix epithelioid carcinoma cell line (HeLa). Various concentrations of gypenosides were individually added to the culture medium of human cervix epithelioid carcinoma cells (HeLa). The N-acetylation of AF was determined by high performance liquid chromatography (HPLC) assaying for the amounts of acetylated 2-aminofluorene (AAF) and nonacetylated 2-aminofluorene (AF). The N-acetylation of AF in the human HeLa cancer cells was suppressed by gypenosides in a dose-dependent manner. The data also demonstrated that gene expression (NAT1 mRNA) of NAT in human cervix epithelioid carcinoma cells (HeLa) was inhibited and decreased by gypenosides. After the incubation of HeLa cells with 30 or 60 microM AF and with or without 350 microg/ml gypenosides cotreatment, DNA was isolated and hydrolyzed to nucleotides, adducted nucleotides were extracted into butanol and analyzed DNA-AF adducts by HPLC. The data demonstrated that gypenosides decrease the levels of DNA-AF adduct formation in HeLa cells.
    關聯: Research Communications in Molecular Pathology and Pharmacology 115-116:157~174
    显示于类别:[中醫學系暨碩博班] 期刊論文

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