Urinary stone disease is a common and high-recurrent disease. There is no ideal medical drug to prevent the recurrence of urinary stone. Therefore, to develop effective medical drugs is a worthwhile issue. The purpose of this study was to investigate the effect of Wu-Ling-San（WLS）on calcium oxalate formation by using crystallization experiments of crystal nucleation, growth, and aggregation in vitro. Then the animal study was followed to investigate the effect of WLS on EG（ethylene glycol）-induced nephrolithiasic rats. The results of crystallization experiments indicated that WLS extract exerted an effect in the inhibition of calcium oxalate crystallization by inhibiting the nucleation and aggregation. The results of animal study revealed WLS could inhibit calcium oxalate crystal deposition effectively in renal tubules of rats fed by EG. Therefore, it may have potential for preventing stone recurrence if further clinical study can be confirmed.
