Differential Expression of GABAA/Benzodiazepine Receptor Subunit mRNAs and Ligand Binding Sites in Mouse Cerebellar Neurons Following In Vivo Ethanol Administration: An Autoradiographic Analysis

CMUR > College of Pharmacy > School of Pharmacy/Master Degree Program, Ph. D program > Journal articles > Item 310903500/3812

Please use this identifier to cite or link to this item: http://ir.cmu.edu.tw/ir/handle/310903500/3812

Title:	Differential Expression of GABAA/Benzodiazepine Receptor Subunit mRNAs and Ligand Binding Sites in Mouse Cerebellar Neurons Following In Vivo Ethanol Administration: An Autoradiographic Analysis
Authors:	吳介信(Chieh-Hsi Wu);(Frostholm A);(DeBlas AL);(Rotter A)
Contributors:	藥學院藥學系;中國附醫醫學研究部
Keywords:	γ-Aminobutyric acid • Alcohol • Chronic ethanol treatment;Acute ethanol treatment;In situ hybridization
Date:	1995-09
Issue Date:	2009-08-20 19:08:41 (UTC+8)
Abstract:	The γ-aminobutyric acidA (GABAA)/benzodiazepine (BZ) receptor is a pentamer composed of subunits belonging to several classes (α1–6, β1–4, γ1–4, δ, and ρ1 and ρ2). In situ hybridization, radioligand autoradiography, and immunocytochemistry were used to examine GABAA/BZ receptor α1, α6, β2, β3, and γ2 subunit expression in murine Purkinje, granule, and deep cerebellar neurons after in vivo ethanol exposure. Chronic ethanol treatment resulted in decreased α1 subunit mRNA expression in each cell type, whereas the expression of α6 and γ2 subunit mRNA levels increased; no changes were observed in the expression of β2 and β3 subunit mRNA. GABA and BZ agonist binding and antibody staining paralleled the changes in mRNA levels. Acute ethanol injection resulted in increased expression of α1 and β3 mRNAs, whereas levels of α6, β2, and γ2 mRNAs remained stable. Our results indicate that, in cerebellar neurons, the expression of specific GABAA/BZ receptor subunit mRNAs, polypeptides, and binding sites is independently regulated by in vivo administration of alcohol. The observed changes were not restricted to any one cerebellar cell type, because subunit expression in Purkinje, granule, and deep cerebellar cells was similarly affected.
Relation:	JOURNAL OF NEUROCHEMISTRY 65(3):1229~1239
Appears in Collections:	[School of Pharmacy/Master Degree Program, Ph. D program] Journal articles

Files in This Item:

File	Size	Format
	58Kb	Unknown	406	View/Open

Loading...