中國醫藥大學機構典藏 China Medical University Repository, Taiwan:Item 310903500/360
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    题名: 中草藥抗發炎作用之篩選暨半枝蓮與Scutellarein抗發炎研究;Studies on the Anti-inflammatory Activity in Selected Traditional Chinese Herbs, Scutellaria rivularis Wallich and Scutellarein
    作者: 廖容君;Jung-Chun Liao
    贡献者: 中國藥學研究所博士班
    关键词: 半枝蓮;一氧化氮;中草藥;鎮痛;抗發炎;抗氧化酵素;Scutellaria rivularis Wallich;nitric oxide;traditional Chinese herbs;analgesic;anti-inflammatory;antioxidant enzymes
    日期: 2006-07-18
    上传时间: 2009-08-10 14:39:58 (UTC+8)
    摘要: 本研究首先從八十七種用於治療抗發炎、解熱及鎮痛之中草藥篩選對LPS與interferon-γ誘導巨噬細胞(RAW264.7)產生NO活性抑制之影響，結果顯示有15種中草藥對LPS與Interferon-γ誘導巨噬細胞(RAW264.7)產生之NO含量有顯著的抑制作用，其抑制率都在80％以上。並就其中活性佳且台灣民間常用的抗癌藥物半枝蓮(Scutellaria rivularis Wallich)進一步以醋酸扭體法、福馬林舔足試驗及λ-carrageenan足蹠腫脹法，探討半枝蓮及其成分scutellarein之鎮痛及抗發炎藥理作用，最後測定λ-carrageenan誘導足蹠腫脹小鼠之腫脹腳掌的MDA及NO含量、肝組織中抗氧化酵素(SOD、GSH-Rd、GSH-Px)活性，以探討scutellarein之抗發炎機轉。
    醋酸扭體法實驗結果發現，口服給予半枝蓮粗抽物與腹腔注射給予scutellarein均可明顯減少小鼠因醋酸所引起疼痛之扭體次數，顯示有鎮痛作用。福馬林舔足試驗實驗結果發現，半枝蓮粗抽物與scutellarein可明顯減少小鼠福馬林誘發後期之舔足反應時間，顯示其具有週邊鎮痛作用。
    λ-carrageenan足蹠腫脹法實驗結果發現，口服給予半枝蓮粗抽物與腹腔注射給予scutellarein可明顯降低λ-carrageenan 誘導的急性足蹠浮腫，顯示半枝蓮粗抽物具有抗發炎作用。
    最後測定λ-carrageenan誘導足蹠腫脹小鼠之腫脹腳掌的MDA及NO含量、肝組織中抗氧化酵素(SOD、GSH-Rd、GSH-Px)活性發現，scutellarein能明顯降低λ-carrageenan所引起之腫脹腳組織NO、MDA濃度及明顯提升肝臟中SOD、GSH-Rd抗氧化酵素的濃度，顯示scutellarein之抗發炎作用與增強自由基的酵素活性，因而減少MDA與NO含量有關。
    綜合上述結果，經由本研究設立的NO assay篩選平台所篩選出來對NO具有抑制活性的半枝蓮與其成分scutellarein，經藥理與生化指數證實確實具有鎮痛與抗發炎作用，期待此簡便的篩選平台能夠提供研究同道們選取抗發炎實驗題材之參考依據。

    In the present study, the effect of eighty-seven species of traditional Chinese herbs on nitric oxide (NO) production by a lipopolysaccharide (LPS) and interferon-γ (IFN-γ) activated murine macrophage-like cell line, RAW 264.7 was investigated. The inhibitory rate of the fifteen species is more than eighty percent. Scutellaria rivularis Wallich, used as anti-cancer drug in Taiwan, showed strong inhibitory effect. Therefore, I investigated the analgesic and anti-inflammatory effects of S. rivularis and its constituent-scutellarein.
    First, we investigated the analgesic effect of S. rivularis and scutellarein by acetic acid-induced writhing response test and formalin test. The anti-inflammatory activity of S. rivularis and scutellarein were determined by the λ- carrageenan induced mice paw edema. The writhing response and carrageenan-induced paw edema showed significantly reduced by treatment with S. rivularis and scutellarein. These results suggested that S. rivularis and scutellarein exerts analgesic and anti-inflammatory activity.
    Scutellarein treatment also caused a significant reduction of the NO and MDA levels in the edema paw, while increasing SOD and GSH-Rx activities in the liver tissue.

    In conclusion, the anti-inflammatory mechanism of scutellarein may be related to an inhibition of the NO and MDA production and the increase in the activities of antioxidant enzymes (SOD, and GSH-Rd). We suggest that scutellarein may be used as a pharmacological agent in preventing and treating disease in which free radical formation is a pathogenic factor.
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