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| 題名: | Genetic variations in the CεmX domain of human membrane-bound IgE |
| 作者: | 萬磊(Lei Wan);(Chen, J.B.);(Chen, H.H.);(Huang, J.);(Yu, H.M.);(Luo, S.F.);蔡輔仁(Fuu-Jen Tsai);(Cheng, T.W.)* |
| 貢獻者: | 中醫系 |
| 關鍵詞: | IgE;CεmX;Single-nucleotide polymorphisms;Alleles;Allergy |
| 日期: | 2010-05 |
| 上傳時間: | 2010-11-19 16:24:53 (UTC+8) |
| 摘要: | The ε chain of membrane-bound IgE (mIgE) is
expressed predominantly as a “long” isoform, containing an
extra segment of 52 amino acid (a.a.) residues, referred to
as CεmX, between the CH4 domain and the C-terminal
membrane-anchoring transmembrane peptide. CεmX
results from an alternative splicing of the ε RNA
transcript at 156-bp upstream of the splicing acceptor site
used by the “short” isoform. Here, based on an analysis of
the CεmX genomic DNA sequences of 320 subjects
residing in Taiwan, we report that single-nucleotide
polymorphisms have been found at two positions, namely,
G/T at #46 and A/G at #93 (along the 156 bp of CεmX),
with the former creating an amino acid change from Val
to Leu at #16 (along the 52 a.a. of CεmX) and the latter
resulting in no change (Gly). Among the 640 CεmX
sequences identified, the previously known 46G93A
allelic form appeared 293 times, the newly discovered
46T93A allelic form (GeneBank accession no.
GU208817) 26 times, and the 46G93G allelic form
(GU208818) 321 times. No 46T93G allelic form was
found. Serum IgE measurements showed that the polymorphisms
did not correlate with the levels of serum IgE.
The anti-CεmX monoclonal antibody, 4B12, could bind
equally well to mIgE.FcL(16V) and mIgE.FcL(16L). While
genetic variation of CεmX of broader populations should
also be investigated, these newly discovered genetic
variants of CεmX in the Taiwanese population do not seem
to affect the feasibility of using an anti-CεmX mAb, such
as 4B12, to target mIgE-expressing B cells. |
| 關聯: | IMMUNOGENETICS 62(5):273-280 |
| 顯示於類別: | [中醫學系暨碩博班] 期刊論文
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