Using a multiplex PCR for identification of Beijing strains of Mycobacterium tuberculosis

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 醫學院 > 臨床醫學研究所 > 期刊論文 > Item 310903500/3390

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题名:	Using a multiplex PCR for identification of Beijing strains of Mycobacterium tuberculosis
作者:	盧章智(Jang-Jih Lu)*;李詩益(Dou HY);杜鴻運(Lee SY);孫俊仁(Sun JR)
贡献者:	醫學院臨床醫學研究所;中國附醫檢驗醫學部
日期:	2008-06
上传时间:	2009-08-20 18:38:14 (UTC+8)
摘要:	The genotype of a Beijing strain of Mycobacterium tuberculosis (MTB) is usually determined by spoligotyping. However, this technique requires special equipment and is time-consuming. In this study, we developed a new multiplex polymerase chain reaction (PCR) to differentiate between Beijing and non-Beijing strains of MTB. A total of 323 MTB isolates were genotyped by both spoligotyping and the novel multiplex PCR. By spoligotyping, 169 (52.3%) isolates were determined to be Beijing strains and the remaining 154 (47.7%) isolates were non-Beijing strains. The multiplex PCR method produced results identical to those of spoligotyping in the identification of Beijing strains of MTB. This method is highly sensitive, specific, and fast. It is also cost-effective and suitable for screening large numbers of samples.
關聯:	EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES 28(1):105~107
显示于类别:	[臨床醫學研究所] 期刊論文

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