Efficient tracking of non-iron-labeled mesenchymal stem cells with serial MRI in chronic stroke rats.

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 醫學院 > 臨床醫學研究所 > 期刊論文 > Item 310903500/3382

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.cmu.edu.tw/ir/handle/310903500/3382

题名:	Efficient tracking of non-iron-labeled mesenchymal stem cells with serial MRI in chronic stroke rats.
作者:	徐偉成;(Chie-Pein Chen);林欣榮(Shinn-Zong Lin);(Yih-Jing Lee);李鴻(Hung Li)
贡献者:	醫學院臨床醫學研究所;中國附醫神經部
关键词:	Vascular disease;Cardiovascular disease;Central nervous system disease;Cerebral disorder;Vertebrata;Mammalia;Rodentia;Monitoring;Bone marrow;Human;Rat;Animal;Chronic;Nuclear magnetic resonance imaging;Iron;Stroke;Cerebrovascular disease;Nervous system diseases
日期:	2007-02
上传时间:	2009-08-20 18:38:10 (UTC+8)
摘要:	Background and Purpose-Although stem cell-based treatments for neurodegenerative diseases have advanced rapidly, there is currently no clinically available method to monitor the fate of transplanted cells in the brain. Methods-To use magnetic resonance imaging for tracking transplanted stem cells in the ischemic rat brain, we used the cellular labeling substance Effectene to transfect a standard contrast agent (Gd-DTPA) into immortalized human bone marrow stromal cells. Results-The transfection efficiency of this method was up to 90%, which is substantially better than pure spontaneous endocytosis or other transfection agents. In addition, cellular uptake of Gd-DTPA in vitro was maintained for >28 days. Therefore, we could follow transplanted stem cell migration and homing into the penumbric area. Using double immunofluorescence, the transplanted cells were seen to differentiate into glial cells, neurons and vascular endothelial cells. Cortical neurochemical activity as evaluated by proton magnetic resonance spectroscopy ('H-MRS) also increased considerably after immortalized human bone marrow stromal cell transplantation. Conclusion-This method of tracking immortalized human bone marrow stromal cells is highly efficient and allows for nontoxic labeling of cells.
關聯:	STROKE 38(2):367~374
显示于类别:	[臨床醫學研究所] 期刊論文

文件中的档案:

档案	大小	格式	浏览次数
	58Kb	Unknown	381	检视/开启

在CMUR中所有的数据项都受到原著作权保护.

TAIR相关文章

数据加载中.....