Aloe-emodin is an interferon-inducing agent with antiviral activity against Japanese encephalitis virus and enterovirus 71

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Title:	Aloe-emodin is an interferon-inducing agent with antiviral activity against Japanese encephalitis virus and enterovirus 71
Authors:	林振文(Lin Cheng Wen)*;萬磊(Lei Wan);林應如(Ying-Ju Lin);林維勇(Wei-Yong Lin)
Contributors:	健康照護學院醫學檢驗生物技術學系;中國附醫檢驗醫學部
Keywords:	Aloe-emodin;Interferon signalling;Japanese encephalitis virus;Enterovirus 71
Date:	2008-09
Issue Date:	2009-08-20 18:29:07 (UTC+8)
Abstract:	In this study, aloe-emodin was identified as a potential interferon (IFN)-inducer by screening compounds from Chinese herbal medicine. Aloe-emodin showed low cytotoxicity to human HL-CZ promonocyte cells and TE-671 medulloblastoma cells and significantly activated interferon-stimulated response element (ISRE) and gamma-activated sequence (GAS)-driven cis-reporting systems. Moreover, aloe-emodin upregulated expression of IFN-stimulated genes such as dsRNA-activated protein kinase and 2′,5′-oligoisoadenylate synthase. Aloe-emodin resulted in significant activation of nitric oxide production. The antiviral activity of aloe-emodin against Japanese encephalitis virus (JEV) and enterovirus 71 (EV71) was evaluated using dose- and time-dependent plaque reduction assays in HL-CZ cells and TE-671 cells. The 50% inhibitory concentration (IC50) of aloe-emodin ranged from 0.50 μg/mL to 1.51 μg/mL for JEV and from 0.14 μg/mL to 0.52 μg/mL for EV71. Aloe-emodin showed clearly potent virus inhibitory abilities and achieved high therapeutic indices, in particular for HL-CZ cells. Therefore, the study demonstrated dose- and time-dependent actions of aloe-emodin on the inhibition of JEV and EV71 replication via IFN signalling responses.
Relation:	INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS 32(4):355~359
Appears in Collections:	[Department of Medical Laboratory Science and Biotechnology ] Journal articles

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