Identification of a novel septin 4 protein binding to human herpesvirus 8 kaposin A protein using a phage display cDNA library

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 醫學院 > 醫學檢驗生物技術學系暨碩士班 > 期刊論文 > Item 310903500/3269

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.cmu.edu.tw/ir/handle/310903500/3269

题名:	Identification of a novel septin 4 protein binding to human herpesvirus 8 kaposin A protein using a phage display cDNA library
作者:	林振文(Cheng-Wen Lin)*;塗炳?(Ping-Feng Tu);蕭乃文(Nai-Wan Hsiao);張清堯(Ching-Yao Chang);萬磊(Lei Wan);林育存(Yu-Tsun Lin);張學偉(Hsueh-Wei Chang)
贡献者:	健康照護學院醫學檢驗生物技術學系;中國附醫檢驗醫學部
关键词:	Human herpesvirus 8;Kaposin A protein;Septin 4;Phage display
日期:	2007-07
上传时间:	2009-08-20 18:29:02 (UTC+8)
摘要:	Human herpesvirus 8 (HHV-8) is associated with the development of Kaposi's sarcoma and several other human malignancies. Kaposin A protein of HHV-8 has been demonstrated as inducing tumorigenic transformation, being responsible for nuclear receptor coactivators in the transforming activity. In this study, a kaposin A-interacting septin 4 variant that contained the unique GDR at the N-terminus and AAALE at the C-terminus was identified using affinity selection of a phage display library. Co-immunoprecipitation and confocal imaging revealed in vitro binding specificity and in vivo co-localization of HHV-8 kaposin A protein to the septin 4 variant. The kaposin A-interacting septin 4 variant induced cell rounding up, activated caspase-3, and up-regulated transcriptional factor NF-κB. By contrast, kaposin A protein showed an antagonistic effect on the biological functions of the septin 4 variant. Therefore, the interaction of kaposin A protein and the septin 4 variant was suggested as playing a possible role in the development of HHV-8-associated malignancies. This study provides insights into the mechanism of the kaposin A protein pathology, in which the interactions of kaposin A protein with cellular proteins might allow alteration of fundamental cellular processes.
關聯:	JOURNAL OF VIROLOGICAL METHODS 1(143):65~72
显示于类别:	[醫學檢驗生物技術學系暨碩士班 ] 期刊論文

文件中的档案:

档案	描述	大小	格式	浏览次数
index.html		0Kb	HTML	333	检视/开启

在CMUR中所有的数据项都受到原著作权保护.

TAIR相关文章

数据加载中.....