A functional epitope determinant on domain III of the Japanese encephalitis virus envelope protein interactions with neutralizing-antibody combining sites.

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Title:	A functional epitope determinant on domain III of the Japanese encephalitis virus envelope protein interactions with neutralizing-antibody combining sites.
Authors:	林振文(Cheng-Wen Lin);(Wu SC.)
Contributors:	健康照護學院醫學檢驗生物技術學系;中國附醫檢驗醫學部
Date:	2003-02
Issue Date:	2009-08-20 18:29:00 (UTC+8)
Abstract:	The envelope (E) protein of Japanese encephalitis virus (JEV) is associated with viral binding to cellular receptors, membrane fusion, and the induction of protective neutralizing-antibody responses in hosts. Most previous studies have not provided detailed molecular information about the spatial configuration of the functional epitopes on domain III of the E protein. Here site-directed mutagenesis was performed to demonstrate that the functional epitope determinants at Ser331 and Asp332 on domain III of the JEV E protein interacted with neutralizing monoclonal antibody (MAb) E3.3. Bacterial expression of the recombinant Fab E3.3 confirmed the molecular interactions of Arg94 in complementary determining region H3 with Ser331 and Asp332 on domain III. This study elucidates the detailed molecular structures of the neutralizing epitope determinants on JEV domain III, which can provide useful information for designing new vaccines.
Relation:	JOURNAL OF VIROLOGY 77(4):2600~2606
Appears in Collections:	[Department of Medical Laboratory Science and Biotechnology ] Journal articles

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