分析中藥在不同粒徑及煎煮方式中其指標成分含量之比較

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題名:	分析中藥在不同粒徑及煎煮方式中其指標成分含量之比較 Effect of different particle sizes and processes of decoction on the extraction of marker components from Traditional Chinese Medicine
作者:	陳妙娟;Miau-Jiuan Chen
貢獻者:	藥學院藥學系碩士班
關鍵詞:	粒徑大小;單煎;合併煎煮;煎煮時間;高效液相層析;particle size;single decoction;mixed decoction;decocted time;HPLC
日期:	2010
上傳時間:	2010-09-29 14:16:15 (UTC+8)
摘要:	傳統中藥湯劑是以中藥飲片為原料進行煎煮，而飲片是否打碎使用，會使藥材接觸溶媒之表面積不同，進而影響其煎出效果，故本研究分析三種中藥（川芎、大黃與當歸）依打碎物顆粒大小，採取單煎、共煎方法以及不同之煎煮時間，使用高效液相色譜測定其指標成分含量，並比較其溶出物之異同關係。藥材飲片經打碎後，依篩孔大小分開，再採取單一或混合加熱煎煮，使用HPLC分析指標成分含量。結果發現大黃在1-4號篩、川芎在2-5號篩以及當歸在6-9號篩中其指標成分萃取量較高。川芎與當歸或大黃合煎可以增加阿魏酸及川芎嗪萃取量；大黃與川芎或當歸合煎可以增加大黃素及大黃酸萃取量；當歸與大黃煎煮會減少當歸之阿魏酸萃取量。由藥材的煎煮時間方面發現，川芎煎煮30 mins、大黃煎煮40 mins、當歸煎煮40 mins以及三個藥材合併煎煮30-40 mins可得到較多含量。本研究顯示，藥材之含量大小與粒徑大小之關係並非一致，藥材在混合煎煮中會增加或抑制其指標成分之溶出，而建議監控煎煮時間可使藥材得到最多溶出量。本研究所採用之HPLC分析方法可提供含此三種藥材之市售固有成方之分析。 Traditionally, the components in Traditional Chinese Medicine (TCM) are extracted by decocting the crude drugs. However, crushing will increase the surface area of the decoction pieces, and thus could increase the efficiency of extraction of the marker components. This study compared analyzes the different particle size, single decoction, mixed decoction, and the different decocted time on the efficiency in extracting the components from the three crude drugs (Chiuanxiong Rhizoma, Rhei Rhizoma and Angelica Sinensis). High performance liquid chromatography (HPLC) was used to compare the amount of marker components and the relationship with these factors. The crude drugs were divided into 9 groups according to sieving sizes NO.1-9 following grounding. Single or mixed decoction and decocted with different decocted time were compared. The results showed that the most efficient particle size was different for each crude drug. The efficient sizes were NO.1-4 for Rhei Rhizoma, NO.2-5 for Angelica Sinensis and NO.6-9 for Chiuanxiong Rhizoma. The amount of marker components in Chiuanxiong Rhizoma could be increased through mixed decoction with the other two crude drugs. The same happened to Rhei Rhizoma, with the exception of ferulic acid, which decreased after Rhei Rhizoma was decocted with Angelica Sinensis. The most efficient decoction time was 40 minutes for Chiuanxiong Rhizoma, 40 minutes for Rhei Rhizoma, 30 minutes for Angelica Sinensis, and 30-40 minutes for mixed decoction. In conclusion, the amount of marker components extracted was not consistent to particle sizes, and the amount of marker components could be increased or decreased by mixed decoction. Through this study, it is also recommended that the decocted time be monitored in order to obtain more active ingredients. Furthermore, the method of HPLC has been effectively used for analyzing the formulas contain the three drugs.
顯示於類別:	[藥學系暨碩博士班] 博碩士論文

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