Cinnamophilin (CINN) isolated from Cinnamomum philippinense, is a strong novel antioxidant and free radical scavenger .It can decrease platelet aggregation and improve the post-stroke neurobehavioral outcome. CINN has the potential to develop into a new drug for treating acute ischemic stroke.
In this study, we use Sprague-Dawley rats for the pharmacokinetic model. They were divided into two groups and administrated via jugular vein injection at dose 40 and 80 mg/kg CINN respectively. Blood and tissue samples were collected at times of 5, 15, 30, 60, 120, 240mins and 20 hrs after intravenous administration. Then CINN and its metabolites in blood or tissue were detected by a high-performance liquid chromatography system.
The results showed that CINN was absorbed and metabolized rapidly in blood and its metabolites had been detected at 20 hours after administration. We found two metabolites of CINN in plasma, and the CINN glucuronides is the major metabolite. Comparison of two different doses of AUC data, we found that the dose-dependent relationship exists in the absorption of CINN. In addition, we found CINN in the brain tissue. It was proved that CINN can cross the blood-brain barrier but could not be detected at 60 min after administration.
The result provides an animal model for the pharmacokinetic study of CINN. It helps us to understand metabolism and distribution of CINN and is useful to modify chemical structure of CINN and increase its activity.
